ABS1063

Anti-phospho-LRP-6 (Thr1479)

from rabbit

• 동의어(들): Low-density lipoprotein receptor-related protein 6
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 종 반응성: human, mouse
• 종 반응성(상동성에 의해 예측): rhesus monkey (based on 100% sequence homology)
• 포장: antibody small pack of 25 μg
• 기술: inhibition assay: suitable (peptide); western blot: suitable
• 동형: IgG
• NCBI 수납 번호: NP_002327
• UniProt 수납 번호: O75581
• 타겟 번역 후 변형: phosphorylation (pThr1479)
• 유전자 정보: human ... LRP6(4040)

설명

일반 설명

Low-density lipoprotein receptor-related protein 6 (UniProt: O75581 also known as LRP-6) is encoded by the LRP6 gene (Gene ID: 4040) in human. LRP-6 is a disulfide-linked homodimeric, single-pass type I transmembrane protein, which is a component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling. LRP-6 is widely co-expressed with LRP5 during embryogenesis and in adult tissues and its levels decrease upon Wnt3A stimulation. It is synthesized with a signal peptide (aa 1-19), which is cleaved off in the mature form. LRP-6 has an extracellular domain (aa 20-1370), a short helical transmembrane domain (aa 1371-1393), and a cytoplasmic tail (aa 1394-1613). LRP-6 contains five PPPSP motifs that play a central role in signal transduction following phosphorylation and activation of Wnt signaling pathway. Dual phosphorylation of cytoplasmic PPPSP motifs sequentially by GSK3 and CK1 is shown to be essential for AXIN1-binding, and subsequent stabilization and activation of beta-catenin via preventing GSK3-mediated phosphorylation of beta-catenin. Upon Wnt signaling, LRP-6 is shown to undergo a cycle of caveolin- or clathrin-mediated endocytosis and plasma membrane location and it undergoes phosphorylation, aggregation, and co-localization with AXIN1 and GSK-3beta at the plasma membrane in LRP-6 signalsomes. It is released from the endoplasmic reticulum following palmitoylation. On two sites near the transmembrane domain. However, in the absence of palmitoylation mono-ubiquitination retains it in the endoplasmic reticulum. Threonine1479 of LRP-6 is phosphorylated by casein kinase I (CK1) and this phosphorylation is facilitated by TMEM198. (Ref.: Liang, J., et al. (2011). Mol. Cell Biol. 31(13); 2577-2590).

특이성

This rabbit polyclonal antibody specifically detects LRP6 phosphorylated on Threonine 1479.

면역원

Epitope: unknown

KLH-conjugated linear peptide corresponding to 10 amino acids surrounding phosphorylated Threonine 1479 of human Low-density lipoprotein receptor-related protein 6 (LRP6).

애플리케이션

Anti-phospho-LRP-6 (Thr1479), Cat. No. ABS1063, is a rabbit polyclonal antibody that detects Low-density lipoprotein receptor-related protein 6 phosphorylated on Thr 1479 and has been tested for use in Western Blotting and Peptide Inhibition Assay.

Research Category
Signaling

Western Blotting Analysis: 1 µg/mL from a representative lot detected phospho-LRP-6 (Thr1479) in membrane fraction extract of 293T cells co-transfected with N-terminal flag-tagged hLRP6 and Xenopus l. Casein Kinase 1g1.

Peptide Inhibition Analysis: A 1:500 dilution from a representative lot was used with membrane fraction extract of 293T cells co-transfected with N-terminal flag tagged hLRP6 and Xenopus l. Casein Kinase 1g1 for peptide block analysis.

품질

Evaluated by Western Blotting in membrane fraction extract obtained from Wnt3a medium treated mouse embryonic fibroblasts.

Western Blotting Analysis: 1 µg/mL of this antibody detected phospho-LRP-6 (Thr1479) in membrane fraction extract obtained from Wnt3a medium treated mouse embryonic fibroblasts.

표적 설명

~220 kDa observed; 180.43 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

물리적 형태

Affinity Purified

Format: Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

기타 정보

Concentration: Please refer to lot specific datasheet.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.