ABN1346

Anti-SHIP1 Antibody

from rabbit, purified by affinity chromatography

• 동의어(들): Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1, hp51CN, Inositol polyphosphate-5-phosphatase of 145 kDa, p150Ship, SH2 domain-containing inositol 5′-phosphatase 1, SH2 domain-containing inositol phosphatase 1, SHIP-1, SHIP1, SIP-145
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 정제법: affinity chromatography
• 종 반응성: human
• 기술: immunohistochemistry: suitable; western blot: suitable
• NCBI 수납 번호: NP_001017915
• UniProt 수납 번호: Q92835
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... INPP5D(3635)

설명

일반 설명

Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1 (EC 3.1.3.86; UniProt Q92835; also known as hp51CN, Inositol polyphosphate-5-phosphatase of 145 kDa, p150Ship, SH2 domain-containing inositol 5′-phosphatase 1, SH2 domain-containing inositol phosphatase 1, SHIP-1, SIP-145) is encoded by the INPP5D (also known as SHIP, SHIP1) gene (Gene ID 3635) in human. SHIP1 and SHIP2 are related phosphatases that play important roles in modulating cell signaling by regulating the phosphorylation state of phosphatidylinositol phosphates (PIPs). While SHIP1 and SHIP2 share a high degree of amino acid conservation, they differ significantly in their cellular expression and receptor recruitment. SHIP1 is primarily confined to cells of the hematopoietic lineage, although it is also expressed by embryonic and mesenchymal stem cells (ESCs and MSCs). SHIP2 is ubiquitously expressed in all cell and tissue types, with especially high levels found in the heart, skeletal muscle, and placenta. SHIP1 functions as s negative regulator in immunoreceptor signaling, in hematopoietic progenitor cell proliferation/survival, and as an inducer of cellular apoptosis. Humna SHIP1 consists of an N-terminal SH2 domain (a.a. 5-101) that mediates interactions with a large number of intracellular signaling proteins. SHIP1 also contains three SH3-interacting regions (a.a. 124-129, 969-974, 1040-1051) characterized by the presence of the PxxP motifs and two NPXY motifs (a.a. 912-915 & 1019-1022) that interact with SH2 domain-containing proteins.

특이성

Immunogen sequence is present in all three spliced isoforms of human SHIP1 reported by UniProt (Q92835).

면역원

Epitope: Near C-terminus

KLH-conjugated linear peptide corresponding to a sequence near the C-terminus of human SHIP1.

애플리케이션

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected SHIP1 in human lymphoma tissue, as well as in normal tonsil and kidney tissue sections.

Research Category
Neuroscience

Research Sub Category
Kinases & Phosphatases

This Anti-SHIP1 Antibody is validated for use in Western Blotting, Immunohistochemistry for the detection of SHIP1.

품질

Evaluated by Western Blotting in Ramos cell lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected SHIP1 in 10 µg of Ramos cell lysate.

표적 설명

~145 kDa observed. Target band size appears larger than the calculated molecular weights of 133.63 kDa (isoform 1), 133.2 kDa (isoform 2), 109.3 kDa (isoform 3) due to posttranslational phosphorylation.

물리적 형태

Affinity Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

기타 정보

Concentration: Please refer to lot specific datasheet.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1