ABF125

Anti-S100A8/MRP-8 Antibody (Azide Free)

serum, from rabbit

• 동의어(들): Protein S100-A8, Calgranulin-A, Chemotactic cytokine CP-10, Leukocyte L1 complex light chain, Migration inhibitory factor-related protein 8, MRP-8, p8, Pro-inflammatory S100 cytokine, S100 calcium-binding protein A8, S100A8/MRP-8
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: serum
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 종 반응성: mouse, human
• 기술: activity assay: suitable; immunohistochemistry: suitable; western blot: suitable
• NCBI 수납 번호: NP_038678
• UniProt 수납 번호: P27005
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... S100A8(6279)

설명

일반 설명

S100A8, also known as Protein S100-A8, Calgranulin-A, Calprotectin L1L subunit, Cystic fibrosis antigen (CFAG), Leukocyte L1 complex light chain, Migration inhibitory factor related protein 8 (MRP-8), p8, S100 calcium binding protein A8, or Urinary stone protein band A, and encoded by the gene S100A8/CAGA/CFAG/MRP8, is an important protein that binds both zinc and calcium and plays an important role in the regulation of inflammatory processes and immune response. Found in complex with its partner S100A9, S100A8 facilitates chemotaxis, fatty acid trafficking, cytoskeleton reorganization, and NAPDPH oxidase activation and other intracellular activities, as well as a host of extracellular induced activities such as extracellular proinflammatory, antimicrobial, oxidant scavenging, and apoptotic activities . Additionally S100A8 can act as a potent autoimmunity amplifier and appears to augment various cancers and their spread when over expressed. S100A8 is widely expressed and used as biomarker in patients with inflammatory diseases and as a cancer marker in multiple forms of cancer.

면역원

Recombinant protein corresponding to mouse S100A8/MRP-8.

애플리케이션

Activity Assay Analysis: A representative lot detected that S100A8/MRP-8 reduces mouse neutrophil accumulation in dorsum-raised air pouchs in the presence of MSU crystals (Ryckman, C., et al. (2003). ARTHRITIS & RHEUMATISM. 48(8):2310–2320).
Immunohistochemistry Analysis: A representative lot detected S100A8/MRP-8 in mouse arthritic paw tissue (Cesaro, A., et al. (2012). PLOS ONE. 7(9):e4578).

Research Category
Inflammation & Immunology

Research Sub Category
Immunoglobulins & Immunology

This Anti-S100A8/MRP-8 Antibody (Azide Free) is validated for use in Western Blotting and Immunohistochemistry and Activity Assay for the detection of S100A8/MRP-8 (Azide Free).

품질

Evaluated by Western Blotting in human spleen tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected S100A8/MRP-8 in 10 µg of human spleen tissue lysate.

표적 설명

~10 kDa observed. Uncharacterized band(s) may appear in some lysates.

물리적 형태

Rabbit polyclonal serum without preservatives.

Unpurified

저장 및 안정성

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable