ABE552

Anti-Alpha-ketoglutarate-dependent dioxygenase FTO Antibody

from rabbit, purified by affinity chromatography

• 동의어(들): Alpha-ketoglutarate-dependent dioxygenase FTO, Fat mass and obesity-associated protein
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 정제법: affinity chromatography
• 종 반응성: human
• 종 반응성(상동성에 의해 예측): monkey (based on 100% sequence homology), sheep (based on 100% sequence homology), ox (based on 100% sequence homology), chimpanzee (based on 100% sequence homology)
• 기술: immunoprecipitation (IP): suitable; western blot: suitable
• NCBI 수납 번호: NP_001073901
• UniProt 수납 번호: Q9C0B1
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... FTO(79068)

설명

일반 설명

Alpha-ketoglutarate-dependent dioxygenase FTO (Fat mass and obesity-associated protein) is a ubiquitous nuclear protein which belongs to the FTO family. It is a dioxygenase enzyme that dealkylates nucleic acids by oxidative demethylation in the presence of oxygen, alpha-ketoglutarate and iron (Fe2+). FTO is highly active on single-stranded RNA molecules with 3-methyluracil residues and on single-stranded DNA containing 3-methylthymine residues, and has relatively low activity on single-stranded DNA containing either 3-methylcytosine or 3-methyladenine. There has been no observed activity on double-stranded DNA. FTO is highly abundant in the hypothalamus, pituitary, and adrenal glands. It plays an important role in energy homeostasis and the regulation of fat accumulation in the body, and may be involved in obesity. Mutated FTO proteins are linked to a syndrome characterized by retarded growth, delayed development, coarse facies and early death.

면역원

KLH-conjugated linear peptide corresponding to human Alpha-ketoglutarate-dependent dioxygenase FTO.

애플리케이션

Immunoprecipitation Analysis: 10 μg from a representative lot immunoprecipitated Alpha-ketoglutarate-dependent dioxygenase FTO from 500 μg of U2OS nuclear extract.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

This Anti-Alpha-ketoglutarate-dependent dioxygenase FTO Antibody is validated for use in western blotting & IP for the detection of Alpha-ketoglutarate-dependent dioxygenase FTO.

품질

Evaluated by Western Blotting in U2OS nuclear extract.

Western Blotting Analysis: 1 μg/mL of this antibody detected Alpha-ketoglutarate-dependent dioxygenase FTO in 10 μg of U2OS nuclear extract.

표적 설명

~58 kDa observed

물리적 형태

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable