추천 제품
생물학적 소스
rabbit
항체 형태
serum
항체 생산 유형
primary antibodies
클론
polyclonal
종 반응성
human, rat, mouse
기술
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBI 수납 번호
UniProt 수납 번호
배송 상태
dry ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... FUNDC1(139341)
일반 설명
FUN14 domain-containing protein 1 (FUNDC1) is an important autophagy partner for mitophagy, the process whereby mitochondria get recycled after damage. FUNDC1 is localized to the outer mitochondrial membrane and widely expressed in all cells and appears to act as an activator of hypoxia-induced mitophagy. FUNDC1 interacts directly with important autophagy markers such as MAP1LC3B and GABARAP. Phosphorylation at Tyr-18 inactivates FUNDC1 mitophagy activation ability, and it is this dephosphorylation following hypoxia that initiates the interaction with MAP1LC3 family proteins and mitophagy. Recent research shows that FUNDC1 expression is controlled via the microRNA miRNA-137, and that this microRNA is critical for mitophagy and mitochondrial recycling.
면역원
Epitope: Sigma transmembrane domain
Recombinant protein corresponding to the Sigma transmembrane domain of Human FUNDC1.
애플리케이션
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Apoptosis - Additional
This Anti-FUNDC1 Antibody is validated for use in Western Blotting and Immunocytochemistry and Immunoprecipitation for the detection of FUNDC1.
Western Blotting Analysis: A representatitive lot of this antibody was used to detect FUNDC1 in A431, C2C12, HEK293, HeLa, HepG2, L6, Mouse brain, NIH/3T3, PC12, PC3, and RAW264.7 cell lysates. This antibody did not detect FUNDC1 in Human placenta, HUVEC, or Jurkat cell lysates.
Western Blotting Analysis: A representatitive lot of this antibody was used to detect puried FUNDC1 protein from bacteria or HeLa cell lysates (Liu, L., et al. (2012) Nature Cell Biology. 14(2):177-185).
Immunocytochemistry Analysis: A representatitive lot of this antibody was used to detect FUNDC1 in HeLa cells (Liu, L., et al. (2012) Nature Cell Biology. 14(2):177-185.
Immunoprecipitation Analysis: A representatitive lot of this antibody was used to co-immunoprecipitate FUNDC1 with endogenous LC3 in HeLa cells that were exposed to normoxic or hypoxic conditions for 24 hrs (Liu, L., et al. (2012) Nature Cell Biology. 14(2):177-185).
Western Blotting Analysis: A representatitive lot of this antibody was used to detect puried FUNDC1 protein from bacteria or HeLa cell lysates (Liu, L., et al. (2012) Nature Cell Biology. 14(2):177-185).
Immunocytochemistry Analysis: A representatitive lot of this antibody was used to detect FUNDC1 in HeLa cells (Liu, L., et al. (2012) Nature Cell Biology. 14(2):177-185.
Immunoprecipitation Analysis: A representatitive lot of this antibody was used to co-immunoprecipitate FUNDC1 with endogenous LC3 in HeLa cells that were exposed to normoxic or hypoxic conditions for 24 hrs (Liu, L., et al. (2012) Nature Cell Biology. 14(2):177-185).
품질
Evaluated by Western Blotting in HepG2 cell lysate.
Western Blotting Analysis: A 1:1000 dilution of this antibody detected FUNDC1 in 10 µg of HepG2 cell lysate.
Western Blotting Analysis: A 1:1000 dilution of this antibody detected FUNDC1 in 10 µg of HepG2 cell lysate.
표적 설명
~17 kDa observed.
Uncharacterized bands may be visitible in some lysates.
Uncharacterized bands may be visitible in some lysates.
물리적 형태
Rabbit Polyclonal serum with 0.05% sodium azide.
Unpurified
저장 및 안정성
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
기타 정보
Concentration: Please refer to lot specific datasheet.
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Mingjie Yang et al.
British journal of pharmacology, 178(4), 964-982 (2020-12-11)
Chronic alcohol consumption contributes to contractile dysfunction and unfavourable geometric changes in myocardium, accompanied by altered autophagy and disturbed mitochondrial homeostasis. The E3 ubiquitin ligase Parkin encoded by PARK2 gene maintains a fundamental role in regulating mitophagy and mitochondrial homeostasis
Panchanan Maiti et al.
International journal of molecular sciences, 20(2) (2019-01-24)
Autophagy and the (PI3K-Akt/mTOR) signaling pathway play significant roles in glioblastoma multiforme (GBM) cell death and survival. Curcumin (Cur) has been reported to prevent several cancers, including GBM. However, the poor solubility and limited bioavailability of natural Cur limits its
Cheng Wang et al.
Nature communications, 12(1), 2616-2616 (2021-05-12)
FUN14 domain-containing protein 1 (FUNDC1) is an integral mitochondrial outer-membrane protein, and mediates the formation of mitochondria-associated endoplasmic reticulum membranes (MAMs). This study aims to determine the contributions of FUNDC1-mediated MAMs to angiogenesis in vitro and in vivo. In cultured
Wen-Qi Ma et al.
Cell death & disease, 11(11), 991-991 (2020-11-19)
Pyruvate dehydrogenase kinase 4 (PDK4) is an important mitochondrial matrix enzyme in cellular energy regulation. Previous studies suggested that PDK4 is increased in the calcified vessels of patients with atherosclerosis and is closely associated with mitochondrial function, but the precise
Kibrom M Alula et al.
Scientific reports, 13(1), 18-18 (2023-01-03)
Autophagy of damaged mitochondria, called mitophagy, is an important organelle quality control process involved in the pathogenesis of inflammation, cancer, aging, and age-associated diseases. Many of these disorders are associated with altered expression of the inner mitochondrial membrane (IMM) protein
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