ABC110

Anti-ELMO1 Antibody

from rabbit, purified by affinity chromatography

• 동의어(들): Engulfment and cell motility protein 1, Protein ced-12 homolog
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 정제법: affinity chromatography
• 종 반응성: human
• 종 반응성(상동성에 의해 예측): bovine (based on 100% sequence homology), mouse (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology)
• 기술: western blot: suitable
• NCBI 수납 번호: NP_055615
• UniProt 수납 번호: Q92556
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... ELMO1(9844)

설명

일반 설명

Engulfment and cell motility protein 1 (ELMO1) is a cytoplasmic protein involved in the clearance of apoptotic cells by phagocytosis. ELMO1 is activated downstream of the phosphatidylserine receptor BAI1. ELMO1 binds to DOCK180 and facilitates its transport to the plasma membrane where DOCK180 activates the GTPase RAC1, possibly via Arf6. RAC1, like other small GTPases, mediate cytoskeleton remodeling and cell motility which is necessary for the internalization of target cells.

특이성

No homology to Rat was found.

This antibody recognizes ELMO1 at the ELMO domain.

면역원

Epitope: ELMO domain

KLH-conjugated linear peptide corresponding to human ELMO1 at the ELMO domain.

애플리케이션

Detect ELMO1 using this Anti-ELMO1 Antibody validated for use in Western Blotting.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

품질

Evaluated by Western Blot in Jurkat cell lysate.

Western Blot Analysis: 0.1 µg/mL of this antibody detected ELMO1 on 10 µg of Jurkat cell lysate.

표적 설명

~78 kDa observed. This antibody exhibits strong affinity, high dilutions are recommended. The calculated molecular weight is 84 kDa, however ELMO1 has been shown as a ~78 kDa band in western blots (Gumienny, T.L., et al. (2001). Cell. 107:27–41.)

물리적 형태

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

분석 메모

Control
Jurkat cell lysate

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable