추천 제품
생물학적 소스
rabbit
Quality Level
항체 형태
purified immunoglobulin
항체 생산 유형
primary antibodies
클론
polyclonal
종 반응성
mouse, human
종 반응성(상동성에 의해 예측)
all
제조업체/상표
Chemicon®
기술
immunohistochemistry: suitable (paraffin)
western blot: suitable
배송 상태
wet ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... NOS1(4842)
일반 설명
The cellular production of highly reactive nitrogen species derived from nitric oxide, such as peroxynitrite, nitrogen dioxide and nitryl chloride, leads to the nitration of tyrosine resides in tissue proteins. The extent of protein nitrotyrosine formation provides an index of the production of reactive nitrogen species and potential cell damage over a period of time. Nitrotyrosine can be measured by amino-acid analysis of protein hydrolysates and detected, estimated semi-quantitatively and located in cells and tissues by immunocytochemical techniques using antibodies directed against the nitrotyrosine hapten.
특이성
Reactivity with other species has not been determined.
Recognizes nitrated tyrosine residues.
면역원
Nitrated KLH.
애플리케이션
Detect Nitrotyrosine using this Anti-Nitrotyrosine Antibody validated for use in IH(P) & WB.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Oxidative Stress
Oxidative Stress
Western Blot:
1:100-1:1,000 dilution of a previous lot was used sucessfully in western blot when tested against nitrated BSA and nitrated ovalbumin.
Immunohistochemistry:
1:50-1:100 on paraffin embedded, 10% neutral buffer formalin fixed mouse liver (animal treated with Zymosan) and normal human cerebellum tissues. The tissues must be pretreated with heat induced epitope retrieval (HIER). Incubation with the AB5411 was for 10 minutes and the reaction was developed with the Chemicon IHCSelect, HRP-DAB kit (Catalog number DAB150).
Optimal working dilutions must be determined by end user.
1:100-1:1,000 dilution of a previous lot was used sucessfully in western blot when tested against nitrated BSA and nitrated ovalbumin.
Immunohistochemistry:
1:50-1:100 on paraffin embedded, 10% neutral buffer formalin fixed mouse liver (animal treated with Zymosan) and normal human cerebellum tissues. The tissues must be pretreated with heat induced epitope retrieval (HIER). Incubation with the AB5411 was for 10 minutes and the reaction was developed with the Chemicon IHCSelect, HRP-DAB kit (Catalog number DAB150).
Optimal working dilutions must be determined by end user.
품질
Immunohistochemistry(paraffin) Analysis:
Nitrotyrosine (AB5411) staining of Mouse Brain (Zymosan), tissue pretreated with citrate buffer, pH 6.0. Polyclonal antibody was diluted to 1:500, using IHC-Select Detection with HRP-DAB. Immunoreactivity is seen in here in hippocampus.
Optimal Staining of Nitrotyrosine Monoclonal: Mouse Brain Treated With Zymosan.
Nitrotyrosine (AB5411) staining of Mouse Brain (Zymosan), tissue pretreated with citrate buffer, pH 6.0. Polyclonal antibody was diluted to 1:500, using IHC-Select Detection with HRP-DAB. Immunoreactivity is seen in here in hippocampus.
Optimal Staining of Nitrotyrosine Monoclonal: Mouse Brain Treated With Zymosan.
결합
Replaces: AB5386
물리적 형태
Format: Purified
Protein A purified
Purified rabbit polyclonal in buffer containing 0.1% sodium azide.
저장 및 안정성
Stable for 1 year at 2-8ºC from date of receipt.
분석 메모
Control
POSITIVE CONTROL: Tyrosine residues can be nitrated directly in situ on tissue sections using peroxynitrite (catalog # 20-107) to create a positive control. After the tissue section is deparaffinized, the slide is covered with a drop of PBS, pH 7.4 into which 10 microliters of peroxynitrite solution are mixed. Care should be taken that the solution maintains a pH of 7.4.
POSITIVE CONTROL: Tyrosine residues can be nitrated directly in situ on tissue sections using peroxynitrite (catalog # 20-107) to create a positive control. After the tissue section is deparaffinized, the slide is covered with a drop of PBS, pH 7.4 into which 10 microliters of peroxynitrite solution are mixed. Care should be taken that the solution maintains a pH of 7.4.
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
NAD(P)H oxidase-derived reactive oxygen species contribute to age-related impairments of endothelium-dependent dilation in rat soleus feed arteries.
Trott, DW; Seawright, JW; Luttrell, MJ; Woodman, CR
Journal of Applied Physiology (1985)
iNOS activity is necessary for the cytotoxic and immunogenic effects of doxorubicin in human colon cancer cells.
De Boo, S; Kopecka, J; Brusa, D; Gazzano, E; Matera, L; Ghigo, D; Bosia, A; Riganti, C
Molecular Cancer null
Hyperglycemia accelerates apparent diffusion coefficient-defined lesion growth after focal cerebral ischemia in rats with and without features of metabolic syndrome.
Tarr, D; Graham, D; Roy, LA; Holmes, WM; McCabe, C; Mhairi Macrae, I; Muir, KW; Dewar, D
Journal of Cerebral Blood Flow and Metabolism null
Ferric carboxymaltose-mediated attenuation of Doxorubicin-induced cardiotoxicity in an iron deficiency rat model.
Toblli, JE; Rivas, C; Cao, G; Giani, JF; Funk, F; Mizzen, L; Dominici, FP
Chemotherapy Research and Practice null
The anti-oxidative, anti-inflammatory, and protective effect of S100A8 in endotoxemic mice.
Sun, Y; Lu, Y; Engeland, CG; Gordon, SC; Sroussi, HY
Molecular Immunology null
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