AB15282

Anti-Cone Arrestin Antibody

Chemicon^®, from rabbit

• 동의어(들): Anti-Arrestin-C
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 분자량: antibody estimated mol wt ~46 kDa
• 정제법: affinity chromatography
• 종 반응성: rat, mouse
• 제조업체/상표: Chemicon^®
• 기술: immunohistochemistry: suitable (paraffin); western blot: suitable
• 동형: IgG
• NCBI 수납 번호: NP_573468
• UniProt 수납 번호: Q9EQP6
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: mouse ... Arr3(170735) ; rat ... Arr3(171107)

설명

일반 설명

Arrestin-C (UniProt: Q9EQP6; also known as Cone arrestin, cArr, Retinal cone arrestin-3) is encoded by the Arr3 gene (Gene ID: 170735) in murine species. Arrestins are a superfamily of multi-functional proteins that that regulate signaling and trafficking of the majority of G-protein-coupled receptors (GPCRs), as well as sub-cellular localization and activity of many other signaling proteins. Arrestin-C, a disulfide-linked, homodimeric protein, is predominantly found in Inner and outer segments, and the inner plexiform regions of the retina. It is expressed in cone photoreceptors and pinealocytes and may contribute to the shut-off mechanisms associated with high acuity color vision. Arrestin-C is an elongated two-domain molecule with overall fold and key inter-domain interactions that hold the free protein in the basal conformation similar to the other subtypes. Several structural elements are reported to contribute to arrestin binding. The C-terminal acidic region serves a regulatory role in controlling arrestin binding selectivity toward the phosphorylated and activated form of a receptor. The basic N-terminal domain directly participates in receptor interaction and serves a regulatory role via intramolecular interaction with the C-terminal acidic region. Also, two centrally localized domains are directly involved in determining receptor binding specificity and selectivity. Mutations in ARR3 gene in humans have been linked to X-lined myopia 26 that is characterized by typical tigroid fundus changes commonly seen in early onset high myopia. (Ref.: Gurevich, VV., et al. (2018). Protein Cell. 9; 986-1003; Xiao, X., et al. (2016). Mol. Vis. 22; 1257-1266).

특이성

This rabbit polyclonal antibody detects Cone Arrestin. It targets an epitope within 12 amino acids from the C-terminal region.

면역원

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to 12 amino acids from the C-terminal region of mouse Cone Arrestin.

애플리케이션

Tested ApplicationsImmunohistochemistry (Paraffin) Analysis: A 1:500 dilution from a representative lot detected Arrestin-C in Rat retina, Mouse retina and Mouse brain tissue sections.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Anti-Cone Arrestin, Cat. No. AB15282, is a rabbit polyclonal antibody that detects Arrestin-C and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Research Category
Neuroscience

Research Sub Category
Sensory & PNS

품질

Evaluated by Western Blotting in Mouse retina tissue lysate.Western Blotting Analysis: A 1:500 dilution of this antibody detected Cone Arrestin in Mouse retina tissue lysate.

표적 설명

~41.9 kDa

물리적 형태

ImmunoAffinity Purified

Purified rabbit polyclonal antibody in buffer containing 0.02 M phosphate buffer, pH 7.6, 0.25 M NaCl, and 0.1% sodium azide.

저장 및 안정성

Recommended storage: +2°C to +8°C.

기타 정보

Concentration: Please refer to lot specific datasheet.

법적 정보

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable