추천 제품
생물학적 소스
rabbit
Quality Level
항체 형태
affinity purified immunoglobulin
항체 생산 유형
primary antibodies
클론
polyclonal
종 반응성
mouse
제조업체/상표
Chemicon®
기술
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable
NCBI 수납 번호
UniProt 수납 번호
배송 상태
dry ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... VCAN(1462)
일반 설명
Versican is a large multi-domain chondroitin sulphate proteoglycan, is secreted by fibroblasts and other types of cells. It has a N-terminal hyaluronic acid (HA) binding domain. This domain has homology with the G1 and G2 domains of aggrecan, (G1 is the hyaluronic acid binding region), to cartilage link protein which stabilizes the binding of aggrecan to hyaluronic acid, and, to the hyaluronate receptor CD44. In addition, versican also has a lectin like domain, a C-terminal compliment repeat, and two EGF-like repeats, all of which can be found in aggrecan., Versican has an apparent molecular mass of ca. 1000 kDa, of which the core contributes 400 kDa from the 2409 amino acids, and the 12 -15 covalently attached chondroitin sulphate chains make up the rest.
특이성
Mouse versican GAG alpha domain
면역원
Epitope: a.a. 535-598 of mouse versican
GST fusion protein containing amino acids 535-598 of mouse versican.
애플리케이션
Immunohistochemistry (formalin fixed, paraffin embedded): 10 μg/mL. Tissues must be treated with Chondroitinase before staining {0.5U/mL in 100mM Tris-HCL pH 7.2-7.4, 30 minutes at room temperature} Then block and stain.
Western blotting: 0.5 μg/mL
Immunoprecipitation
Optimal working dilutions must be determined by the end user.
Western blotting: 0.5 μg/mL
Immunoprecipitation
Optimal working dilutions must be determined by the end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
ECM Proteins
ECM Proteins
This Anti-Versican Antibody, a.a. 535-598 of mouse versican is validated for use in IP, WB, IH(P) for the detection of Versican.
물리적 형태
Format: Purified
Immunoglobulin was purified by affinity chromatography and is presented in PBS with no preservative Anti-GST has not been adsorbed.
저장 및 안정성
Maintain at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles.
분석 메모
Control
Immunohistochemistry Positive control: surrounding cartilage, neural tube / brainWestern blotting positive control: newborn mouse brain
Immunohistochemistry Positive control: surrounding cartilage, neural tube / brainWestern blotting positive control: newborn mouse brain
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Sabrina Hsiung et al.
Scientific reports, 8(1), 7530-7530 (2018-05-16)
The mechanisms responsible for macrovascular complications in diabetes remain to be fully understood. Recent studies have identified impaired vascular repair as a possible cause of plaque vulnerability in diabetes. This notion is supported by observations of a reduced content of
Expression of a novel versican variant in dorsal root ganglia from spared nerve injury rats.
Oliver Bogen et al.
Molecular pain, 15, 1744806919874557-1744806919874557 (2019-08-21)
Kelly L Short et al.
Journal of molecular endocrinology, 64(3), 155-164 (2020-01-21)
Glucocorticoid (GC) signaling via the glucocorticoid receptor (GR) is essential for lung maturation in mammals. Previous studies using global or conditional mouse model knockouts of the GR gene have established that GR-mediated signaling in the interstitial mesenchyme of the fetal
Sumeda Nandadasa et al.
Matrix biology plus, 10, 100064-100064 (2021-07-02)
Two inherent challenges in the mechanistic interpretation of protease-deficient phenotypes are defining the specific substrate cleavages whose reduction generates the phenotypes and determining whether the phenotypes result from loss of substrate function, substrate accumulation, or loss of a function(s) embodied
Simon J Foulcer et al.
Methods in molecular biology (Clifton, N.J.), 1229, 587-604 (2014-10-19)
Versican is a widely distributed chondroitin sulfate proteoglycan that forms large complexes with the glycosaminoglycan hyaluronan (HA). As a consequence of HA binding to its receptor CD44 and interactions of the versican C-terminal globular (G3) domain with a variety of
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