676602

PhosphoDetect Anti-VASP (pSer²³⁹) Mouse mAb (16C2)

lyophilized, clone 16C2, Calbiochem^®

• 동의어(들): Anti-Vasodilator-stimulated Phosphoprotein, Activated
• UNSPSC 코드: 12352203

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: 16C2, monoclonal
• 형태: lyophilized
• 포함: ≤0.1% sodium azide as preservative
• 종 반응성: mouse, human
• 제조업체/상표: Calbiochem^®
• 저장 조건: OK to freeze
• 동형: IgG1
• 배송 상태: ambient
• 저장 온도: −20°C
• 타겟 번역 후 변형: phosphorylation (pSer239/pSer239)

설명

일반 설명

Mouse monoclonal antibody purified from serum-free cell culture supernatant by thiophilic adsorption and size exclusion chromatography. Recognizes the ~46-50 kDa VASP protein phosphorylated at Ser²³⁹.

Recognizes the ~46-50 kDa VASP protein phosphorylated at Ser²³⁹.

This PhosphoDetect Anti-VASP (pSer²³⁹) Mouse mAb (16C2) is validated for use in ELISA, Immunoblotting, ICC, IP for the detection of VASP (pSer²³⁹).

면역원

Human

a synthetic phosphopeptide corresponding to amino acids surrounding the Ser²³⁹ phosphorylation site of human VASP, conjugated to KLH

애플리케이션

ELISA (0.05 µg/ml)

Immunoblotting (0.1 µg/ml, chemiluminescence; 1 µg/ml, colorimetric)

Immunocytochemistry (1-10 µg/ml)

Immunoprecipitation (1-10 µg/10⁶ vanadate-treated A431 cells)

경고

Toxicity: Standard Handling (A)

물리적 형태

Lyophilized from 2X PBS, PEG, sucrose.

재구성

Reconstitute with 1 ml of dH₂O. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot, flash-freeze in liquid nitrogen, and freeze (-70°C) for long-term storage. Stock solutions are stable for up to 3 months at 4°C or for up to 1 year at -70°C. Thaw aliquots at 37°C. Avoid freeze/thaw cycles of solutions.

분석 메모

Positive Control
Vanadate-treated A431 cells or 8-pCPT-treated platelets

기타 정보

Does not recognize the non-phosphorylated form of VASP. Suitable for monitoring the phosphorylation state of VASP Ser²³⁹ as well as PKA/PKG activity. For immunocytochemistry, SNP or 8-pCPT-cGMP treated platelets are fixed with 4% paraformaldehyde in PBS for 15 min. on ice, then permeabilized with 0.2% Triton^™ X-100 detergent in PBS for 10 min at room temperature. For immunoblotting, block with 1% hemoglobin in PBS, 0.3% Triton X-100 detergent, and 0.05% Tween-20 detergent. Variables associated with assay conditions will dictate the proper working dilution.

Reinhard, M., et al. 1999. In: Guidebook to the Cytoskeletal and Motor Proteins (Eds. Kreis, T., and Vale, R.), Oxford University Press, Oxford, 168.
Schwarz, U.R., et al. 1999. Thromb. Haemost.82, 1145.
Smolenski, A., et al. 1998. J. Biol. Chem.273, 20029.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable