616394

TPEN

• 동의어(들): TPEN, N,N,Nʹ,Nʹ- tetrakis-(2-Pyridylmethyl)ethylenediamine
• 실험식(Hill 표기법): C₂₆H₂₈N₆
• CAS Number: 16858-02-9
• Molecular Weight: 424.54
• MDL number: MFCD00036918
• UNSPSC 코드: 12352116
• NACRES: NA.25

속성

• 분석: ≥97% (titration)
• Quality Level: 100
• 형태: solid
• 반응 적합성: reagent type: chelator
• 제조업체/상표: Calbiochem^®
• 저장 조건: OK to freeze
• 색상: tan
• solubility: ethanol: 10 mg/mL; 0.1 M phosphate pH 7.0: 25 μM; DMSO: soluble
• 배송 상태: ambient
• 저장 온도: 15-25°C
• InChI: 1S/C26H28N6/c1-5-13-27-23(9-1)19-31(20-24-10-2-6-14-28-24)17-18-32(21-25-11-3-7-15-29-25)22-26-12-4-8-16-30-26/h1-16H,17-22H2
• InChI key: CVRXLMUYFMERMJ-UHFFFAOYSA-N

설명

일반 설명

Lipid-soluble heavy metal chelator that crosses artificial and natural membranes. Auxiliary reagent in certain techniques used for measuring free Ca²⁺ in cells. Has an extremely high affinity for heavy metals but a low affinity for Ca²⁺ and Mg²⁺. Induces DNA fragmentation when chelated with Zn²⁺ and causes apoptosis in thymocytes. Strips heavy metals from liposome-trapped QUIN 2. Shown to be useful in extracting Aβ depostis from Alzheimer′s disease brain tissue.

Lipid-soluble heavy metal chelator that crosses artificial and natural membranes. Auxiliary reagent in certain techniques used for measuring free Ca²⁺ levels in cells. Also useful in extracting Aβ amyloid deposits from Alzheimer’s disease brain tissue. Has extremely high affinity for heavy metals but low affinity for Ca²⁺ and Mg²⁺. Induces DNA fragmentation when chelated with Zn²⁺ and induces apoptosis in thymocytes. Strips heavy metals from liposome-trapped QUIN 2 (Cat. No. 551826).

애플리케이션

• Poly(ADP-ribosyl)ation of p53 contributes to TPEN-induced neuronal apoptosis.: This study investigates the role of poly(ADP-ribosyl)ation of the tumor suppressor protein p53 in neuronal apoptosis induced by TPEN, highlighting mechanisms of cell death in neurodegenerative diseases (Kim HL et al., 2015).


• Essential role of p53 in TPEN-induced neuronal apoptosis.: This article explores the critical involvement of the p53 protein in apoptosis initiated by TPEN in neuronal cells, offering insights into potential therapeutic targets for neuroprotection (Ra H et al., 2009).


• Clioquinol induces autophagy in cultured astrocytes and neurons by acting as a zinc ionophore.: Demonstrates how Clioquinol, similar to TPEN, functions as a zinc ionophore to induce autophagy, particularly in neurological contexts, which may be relevant for understanding TPEN′s mechanisms (Park MH et al., 2011).

경고

Toxicity: Standard Handling (A)

재구성

Following reconstitution, aliquot and freeze (-20°C) under an inert gas. Alternately, acidify (pH ≤3) with HCl for long term storage. Stock solutions are stable for up to 3 months at -20°C.

기타 정보

Fonte, J., et al. 2001. J. Alzheimer’s Dis. 3, 209.
Cherny, R.A., et al. 1999. J. Biol. Chem. 274, 23223.
Aballay, A., et al. 1995. Biochem. J.312, 919.
Jiang, S., et al. 1995. Lab. Invest.73, 111.
Sheridan, R.E. and Deshpande, S.S. 1995. Toxicon33, 539.
McCabe, M.J., et al. 1993. Lab. Invest. 69, 101.
Hinkle, P.M., et al. 1992. J. Biol. Chem.267, 25553.
Baba, A., et al. 1991. Brain Res. 557, 103.
Forbes, I.J., et al. 1991. Exp. Cell Res. 195, 224.
Csermely, P. and Somogyi, J. 1989. J. Cellular Physiol.138, 593.
Raspe, E., et al. 1989. Eur. J. Pharmacol.163, 345.
Arslan, L.P., et al. 1985. J. Biol. Chem.260, 2719.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable