574795

TBE Buffer, 10X, Molecular Biology Grade

A 10X concentrate that can be diluted to a 1X solution containing 89 mM Tris, 89 mM boric acid, 2 mM EDTA, pH ~8.3.

• 동의어(들): Tris-Borate-EDTA Buffer
• UNSPSC 코드: 41105319
• NACRES: NA.25

속성

• Grade: Molecular Biology
• 형태: liquid
• 제조업체/상표: Calbiochem^®
• 저장 조건: OK to freeze
• 외래 활성: DNase, RNase, protease, none detected
• 배송 상태: ambient
• 저장 온도: 15-25°C

설명

일반 설명

A 10X concentrate that can be diluted to a 1X solution containing 89 mM Tris, 89 mM boric acid, 2 mM EDTA, pH ~8.3.

Tris, borate, and ethylenediaminetetraacetic acid (TBE) buffer is used to perform agarose gel electrophoresis of DNA and RNA. It consists of a weak acid that exists in neutral and anionic forms (e.g., COOH and COO^- species). Tris is a weak base, which occurs in either neutral or cationic forms (Tris-NH₂ and Tris-NH₃⁺). These ions maintain a low conductivity medium, transmit the electrical current, and buffer the pH. Ethylenediaminetetraacetic (EDTA) although not essential is added as a preventative since it chelates Mg²⁺ ions and inactivates potential DNA nucleases. TBE allows an effective resolution of DNA fragments and slightly improves the separation of smaller fragments. It also stabilizes nucleic acids against enzymatic degradation.

애플리케이션

TBE Buffer, 10X, Molecular Biology Grade-Calbiochem has been used as a component of polyacrylamide sequencing gels for DNA electrophoresis using amplified fragment length polymorphism (AFLP).

경고

Toxicity: Irritant (B)

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H360FD
• 예방조치 성명서: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Hazard Classifications: Repr. 1B
• Storage Class Code: 6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable