분석
≥95% (SDS-PAGE)
Quality Level
형태
lyophilized
제조업체/상표
Calbiochem®
저장 조건
OK to freeze
불순물
≤1 EU/μg Endotxin
배송 상태
ambient
저장 온도
−20°C
일반 설명
Recombinant, mouse secreted frizzled related protein 3 (sFRP-3) consisting of amino acids Ala 33-Ser323, fused at the N-terminus to a His•Tag sequence and expressed in NSO-derived mouse myeloma cells. sFRP-3 is a soluble antagonist of Wnt signaling. It prohibits interactions with Frizzled receptors by binding directly to Wnt proteins.
생화학적/생리학적 작용
Proliferation Assay:
ED₅₀ = 1.6-6 µg/ml as measured by inhibition of proliferation of MC3T3-E1 mouse osteoblastic cells.
Functional ELISA:
50% optimal binding response = 150-250 ng/ml as measured by binding ro rmWnt-3a (500 ng/ml).
ED₅₀ = 1.6-6 µg/ml as measured by inhibition of proliferation of MC3T3-E1 mouse osteoblastic cells.
Functional ELISA:
50% optimal binding response = 150-250 ng/ml as measured by binding ro rmWnt-3a (500 ng/ml).
경고
Toxicity: Standard Handling (A)
물리적 형태
Lyophilized from 0.2 µm filtered PBS, BSA.
재구성
Following reconstituion refrigerate (4°C) for short term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Reconstitute with sterile PBS contanining ≥1% human or bovine serum albumin. Please refer to the vial label for lot-specific reconstitution volume.
기타 정보
Hoang, et al. 1996. J. Biol. Chem.271, 26131.Leyns, et al. 1997 Cell88, 747.Wang, et al. 1997 Cell88, 757.Mayr, et al. 1997 Mech. Dev.63, 109.Rattner, et al. 1997. PNAS94, 2859.
법적 정보
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
11 - Combustible Solids
WGK
WGK 2
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
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