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Merck
모든 사진(1)

주요 문서

527460

Sigma-Aldrich

PhosphoDetect Anti-PKR (pThr⁴⁵¹) Rabbit pAb

liquid, Calbiochem®

동의어(들):

Anti-Double-stranded RNA-dependent Protein Kinase, Phospho-Specific (Thr⁴⁵¹)

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About This Item

UNSPSC 코드:
12352203
NACRES:
NA.41

생물학적 소스

rabbit

Quality Level

항체 생산 유형

primary antibodies

클론

polyclonal

양식

liquid

포함

≤0.1% sodium azide as preservative

종 반응성

human, mouse

제조업체/상표

Calbiochem®

저장 조건

OK to freeze
avoid repeated freeze/thaw cycles

동형

IgG

배송 상태

wet ice

저장 온도

−70°C

타겟 번역 후 변형

phosphorylation (pThr451)

유전자 정보

human ... EIF2AK2(5610)

일반 설명

Note: 1 T = 1 test. Sufficient for 10 Western miniblots.
Rabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phophorylation site, followed by immunoaffinity chromatography. Recognizes the ~65-68 kDa PKR protein phosphorylated at Thr451.
Recognizes the ~65-68 kDa PKR protein phosphorylated at Thr451.
This PhosphoDetect Anti-PKR (pThr⁴⁵¹) Rabbit pAb is validated for use in Immunoblotting, Immunohistochemistry for the detection of PKR (pThr⁴⁵¹).

면역원

a synthetic phosphopeptide corresponding to amino acids surrounding the Thr⁴⁵¹ phosphorylation site of human PKR (double-stranded RNA-dependent protein kinase)

애플리케이션

Immunoblotting (1:1000)

Immunohistochemistry (see comments)

경고

Toxicity: Standard Handling (A)

물리적 형태

In Dulbecco′s PBS (without Mg+2 or Ca+2), 0.1% BSA, 50% glycerol, pH 7.3.

재구성

Following initial thaw, aliquot and freeze (-70°C).

분석 메모

Positive Control
IFNγ-stimulated HeLa cells.

기타 정보

Cuddihy, A.R., et al.1999. Oncogene8, 2690.
Srivastava, S.P., et al. 1998. J. Biol. Chem.273, 2416.
Clemens, M.J. 1997. Int. J. Biochem. Cell Biol.29, 945.
When used at a 1:1000 dilution there is sufficient antibody for 10 blots at 10 ml/blot. This antibody has also been reported to work for immunohistochemistry. Variables associated with assay conditions will dictate the optimal dilution.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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