생물학적 소스
rabbit
Quality Level
항체 생산 유형
primary antibodies
클론
polyclonal
양식
liquid
포함
≤0.1% sodium azide as preservative
종 반응성
human, mouse
제조업체/상표
Calbiochem®
저장 조건
OK to freeze
avoid repeated freeze/thaw cycles
동형
IgG
배송 상태
wet ice
저장 온도
−70°C
타겟 번역 후 변형
phosphorylation (pThr451)
유전자 정보
human ... EIF2AK2(5610)
일반 설명
Note: 1 T = 1 test. Sufficient for 10 Western miniblots.
Rabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phophorylation site, followed by immunoaffinity chromatography. Recognizes the ~65-68 kDa PKR protein phosphorylated at Thr451.
Recognizes the ~65-68 kDa PKR protein phosphorylated at Thr451.
This PhosphoDetect Anti-PKR (pThr⁴⁵¹) Rabbit pAb is validated for use in Immunoblotting, Immunohistochemistry for the detection of PKR (pThr⁴⁵¹).
면역원
a synthetic phosphopeptide corresponding to amino acids surrounding the Thr⁴⁵¹ phosphorylation site of human PKR (double-stranded RNA-dependent protein kinase)
애플리케이션
Immunoblotting (1:1000)
Immunohistochemistry (see comments)
Immunohistochemistry (see comments)
경고
Toxicity: Standard Handling (A)
물리적 형태
In Dulbecco′s PBS (without Mg+2 or Ca+2), 0.1% BSA, 50% glycerol, pH 7.3.
재구성
Following initial thaw, aliquot and freeze (-70°C).
분석 메모
Positive Control
IFNγ-stimulated HeLa cells.
IFNγ-stimulated HeLa cells.
기타 정보
Cuddihy, A.R., et al.1999. Oncogene8, 2690.
Srivastava, S.P., et al. 1998. J. Biol. Chem.273, 2416.
Clemens, M.J. 1997. Int. J. Biochem. Cell Biol.29, 945.
Srivastava, S.P., et al. 1998. J. Biol. Chem.273, 2416.
Clemens, M.J. 1997. Int. J. Biochem. Cell Biol.29, 945.
When used at a 1:1000 dilution there is sufficient antibody for 10 blots at 10 ml/blot. This antibody has also been reported to work for immunohistochemistry. Variables associated with assay conditions will dictate the optimal dilution.
법적 정보
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
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