506114

p34^cdk1/Cyclin B, Human, Recombinant, S. frugiperda

• UNSPSC 코드: 12352202
• NACRES: NA.77

속성

• 형태: liquid
• Quality Level: 100
• 특이 활성도: ≥20,000 U/mL
• 제조업체/상표: Calbiochem^®
• 저장 조건: OK to freeze; avoid repeated freeze/thaw cycles
• 외래 활성: Protease, phosphatase, none detected
• 배송 상태: wet ice
• 저장 온도: −70°C

설명

일반 설명

Recombinant, human p34^Cdk1/cyclin B expressed in S. frugiperda insect cells. This kinase is a major regulator of the eukaryotic cell cycle. As a monomer, Cdk1 is inactive and requires association with cyclin B and phosphorylation of Thr¹⁶¹ to form an active complex. MW: 34000(p34^Cdk1) and 55000(cyclinB).

Recombinant, human p34^Cdk1/cyclin B expressed in S. frugiperda insect cells. This kinase is a major regulator of the eukaryotic cell cycle. As a monomer, p34^cdc2 is inactive and requires association with cyclin B to form an active complex.

경고

Toxicity: Standard Handling (A)

단위 정의

One unit is defined as the amount of enzyme that catalyzes the transfer of 1 pmol of phosphate to histone H1 (10 µM) in 1 min at 30°C, pH 7.5.

물리적 형태

In 100 mM NaCl, 50 mM HEPES, 1 mM DTT, 100 µM EDTA, 50% glycerol, 0.01% BRIJ^® 35 Detergent, pH 7.5.

재구성

Following initial thaw, aliquot and freeze (-70°C).

기타 정보

Kobayashi, H., et al. 1994. J. Biol. Chem. 269, 29153.
Atherton-Fessler, S., et al. 1993. Mol. Cell. Biol. 13, 1675.

법적 정보

Brij is a registered trademark of Croda International PLC

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable