재조합
expressed in E. coli
Quality Level
분석
≥95% (SDS-PAGE)
형태
liquid
특이 활성도
≥5500 units/mg protein
제조업체/상표
Calbiochem®
저장 조건
OK to freeze
avoid repeated freeze/thaw cycles
외래 활성
Proteases, none detected
배송 상태
wet ice
저장 온도
−70°C
일반 설명
Recombinant, human MAP Kinase 2 fused to a His•Tag sequence and expressed in E. coli. Highly active form produced by phosphorylation of the purified MAP Kinase in vitro with MEK1. Suitable for labeling MAP Kinase substrates.
Recombinant, human MAP Kinase 2 fused to a His•Tag sequence and expressed in E. coli. Highly active form produced by phosphorylation of the purified MAP kinase in vitro with MEK1. Suitable for labeling MAP Kinase substrates.
경고
Toxicity: Standard Handling (A)
단위 정의
One unit is defined as the amount of enzyme that will catalyze the transfer of 1.0 pmol phosphate to myelin basic protein (MBP) per min at 30°C.
물리적 형태
In 150 mM NaCl, 50 mM Tris, 1 mM DTT, 50% glycerol, pH 8.5.
재구성
Following initial thaw, aliquot and freeze (-70°C).
기타 정보
Lew, J. 2003. Biochemistry42, 849.
Johnson, G.L., and Lapadat, R. 2002. Science298, 1911.
Koistinaho, M., and Koistinaho, J. 2002. Glia40, 175.
Johnson, G.L., and Lapadat, R. 2002. Science298, 1911.
Koistinaho, M., and Koistinaho, J. 2002. Glia40, 175.
법적 정보
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Milla Koistinaho et al.
Glia, 40(2), 175-183 (2002-10-16)
Although microglial cells are thought to play a beneficial role in the regeneration and plasticity of the central nervous system (CNS), recent studies have indicated that at least some molecules released by microglia may be harmful in acute brain insults
Gary L Johnson et al.
Science (New York, N.Y.), 298(5600), 1911-1912 (2002-12-10)
Multicellular organisms have three well-characterized subfamilies of mitogen-activated protein kinases (MAPKs) that control a vast array of physiological processes. These enzymes are regulated by a characteristic phosphorelay system in which a series of three protein kinases phosphorylate and activate one
John Lew
Biochemistry, 42(4), 849-856 (2003-01-29)
Protein kinases constitute one of the largest enzyme families encoded by the human genome. Owing to their critical role in virtually all aspects of signal transduction, protein kinases have evolved stringent mechanisms for their regulation, which classically falls into two
Single-Molecule Force Spectroscopy on the N2A Element of Titin: Effects of Phosphorylation and CARP.
Thomas Lanzicher et al.
Frontiers in physiology, 11, 173-173 (2020-04-08)
Titin is a large filamentous protein that forms a sarcomeric myofilament with a molecular spring region that develops force in stretched sarcomeres. The molecular spring has a complex make-up that includes the N2A element. This element largely consists of a
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