생물학적 소스
rabbit
Quality Level
항체 형태
purified antibody
항체 생산 유형
primary antibodies
클론
polyclonal
형태
liquid
포함
≤0.1% sodium azide as preservative
종 반응성
Xenopus, Tetrahymena sp., human, Drosophila, Caenorhabditis elegans
제조업체/상표
Calbiochem®
저장 조건
OK to freeze
avoid repeated freeze/thaw cycles
동형
IgG
저장 온도
−20°C
타겟 번역 후 변형
phosphorylation (pSer10)
유전자 정보
human ... H3C1(8350)
일반 설명
Protein A purified rabbit polyclonal antibody. Recognizes the ~17 kDa histone H3 protein phosphorylated at Ser10.
Recognizes the ~17 kDa histone H3 protein phosphorylated at Ser10.
This PhosphoDetect Anti-Histone H3 (pSer¹⁰) (7-20) Rabbit pAb is validated for use in Immunoblotting, ICC, IHC, IP for the detection of Histone H3 (pSer¹⁰) (7-20).
면역원
Human
a synthetic phosphopeptide [ARKpSTGGKAPRKQL(C)] corresponding to amino acids 7-20 surrounding the Ser¹⁰ phosphorylation site of human histone H3, conjugated to KLH
애플리케이션
Immunoblotting (0.5 µg/ml)
Immunocytochemistry (5 µg/ml)
Immunohistochemistry (see comments)
Immunoprecipitation (4 µg/10 µg acid-extracted proteins from colcemid-arrested HeLa cells)
Immunocytochemistry (5 µg/ml)
Immunohistochemistry (see comments)
Immunoprecipitation (4 µg/10 µg acid-extracted proteins from colcemid-arrested HeLa cells)
경고
Toxicity: Standard Handling (A)
물리적 형태
In 150 mM NaCl, 100 mM Tris-glycine, pH 7.4.
재구성
Following initial thaw, aliquot and freeze (-20°C).
분석 메모
Positive Control
Acid-extracted proteins from colcemid-arrested HeLa cells
Acid-extracted proteins from colcemid-arrested HeLa cells
기타 정보
Ajiro, K., et al. 1996. J. Biol. Chem.271, 13197.
Chadee, D.N., et al. 1995. J. Biol. Chem.270, 20098.
Mahadevan, L.C., et al. 1991. Cell65, 775.
Chadee, D.N., et al. 1995. J. Biol. Chem.270, 20098.
Mahadevan, L.C., et al. 1991. Cell65, 775.
For immunocytochemistry, colcemid-arrested HeLa cells were fixed with 1% paraformaldehyde. Also detects histone H3 in Tetrahymena micronuclei. The antibody has been reported to detect mitotic cells in Xenopus, Caenorhabditis elegans, and Drosophila embryos. Does not cross-react with unphosphorylated histone H3. This antibody has also been reported to work for immunohistochemistry. Variables associated with assay conditions will dictate the optimal working dilution.
법적 정보
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
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