376816

Hexokinase II VDAC Binding Domain Peptide, Cell-Permeable

A cell-permeable peptide analog of Hexokinase II VDAC binding domain peptide.

• 동의어(들): Hexokinase II VDAC Binding Domain Peptide, Cell-Permeable, HXK2VBD-cpm, H- RQIKIWFQNRRMKWKK-MIASHLLAYFFTELN-NH₂
• 실험식(Hill 표기법): C₁₈₈H₂₉₁N₅₃O₄₀S₂
• Molecular Weight: 3997.78
• UNSPSC 코드: 12352202
• NACRES: NA.77

속성

• Quality Level: 100
• 분석: ≥95% (HPLC)
• 형태: lyophilized solid
• 제조업체/상표: Calbiochem^®
• 저장 조건: OK to freeze; desiccated (hygroscopic); protect from light
• 색상: white
• solubility: water: 1 mg/mL
• 배송 상태: wet ice
• 저장 온도: −20°C

설명

일반 설명

A cell-permeable peptide analog of Hexokinase II VDAC binding domain (HXK2VBD) peptide (Cat. No. 376815). The internalization sequence of the Antennapedia homeoprotein is fused to the N-terminal methionine of the HXK2VBD peptide. Has been shown to cause the complete release of hexokinase II (HXK2) from the mitochondria and subsequent translocation to the cytosol in HeLa cells (100% release at 100 µM). Does not induce Bax translocation or cytochrome c release. Also shown to markedly sensitize cells to Bax-induced cytochrome c release. Also increases the sensitivity of cells to apoptosis in combination with the Bax-dependent apoptosis inducer, Indomethicin (Cat. No. 405268).

A cell-permeable peptide analog of Hexokinase II VDAC binding domain peptide (Cat. No. 376815). The internalization domain of the Antennapedia homeoprotein is fused to the methionine amino terminal. Shown to completely detach and translocate HXK2 from mitochondria to the cytosol in HeLa cells at 100 µM. Does not induce Bax translocation or cytochrome c release when used alone. However, it markedly sensitizes cells to cytochrome c release and to the induction of apoptosis when used in combination with a Bax-dependent apoptosis inducer, Indomethacin (Cat. No. 405268).

생화학적/생리학적 작용

Cell permeable: yes

Primary Target
Detach and translocate HXK2 from mitochondria to the cytosol

Product does not compete with ATP.

Reversible: no

포장

Packaged under inert gas

경고

Toxicity: Standard Handling (A)

서열

H-Arg-Gln-Ile-Lys-Ile-Trp-Phe-Gln-Asn-Arg-Arg-Met-Lys-Trp-Lys-Lys-Met-Ile-Ala-Ser-His-Leu-Leu-Ala-Tyr-Phe-Phe-Thr-Glu-Leu-Asn-NH₂

기타 정보

Pastorino, J.G., et al. 2002. J. Biol. Chem.277, 7610.
Holinger, E.P., et al. 1999. J. Biol. Chem.274, 13298.
Sui, D., and Wilson, J.E. 1997. Arch. Biochem. Biophys.345, 111.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable