추천 제품
재조합
expressed in insect cells (S. frugiperda)
Quality Level
분석
≥95% (SDS-PAGE)
형태
solid
제조업체/상표
Calbiochem®
저장 조건
OK to freeze
불순물
<1.0 EU/μg protein Endotoxin
배송 상태
ambient
저장 온도
−20°C
일반 설명
Dkk-2, Mouse, Recombinant, consists of amino acids 26-259 and expressed in S. frugiperda 21 cells with a C-terminal His•Tag sequence.
Recombinant, mouse Dickkopf related protein 2 (Dkk-2) consisting of amino acids 26-259 and expressed in S. frugiperda 21 cells with a C-terminal His•Tag sequence. Dkk proteins are expressed in mesenchymal tissues and control epithelial transformations.
생화학적/생리학적 작용
Inhibition assay: Inhibits WNT-3a-induced alkaline phosphatase production in MC3T3-E1 mouse osteoblastic cells in the presence of BMP-2.
Competetive Binding Assay:
Inhibits 50% binding of biotinylated rhDkk-1 (200 ng/ml) to immobilized rmKremen (4 µg/ml) at a concentration of 5-15 µg/ml.
Competetive Binding Assay:
Inhibits 50% binding of biotinylated rhDkk-1 (200 ng/ml) to immobilized rmKremen (4 µg/ml) at a concentration of 5-15 µg/ml.
경고
Toxicity: Standard Handling (A)
물리적 형태
Lyophilized from 0.2 µm filtered PBS, BSA.
재구성
Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Reconstitute to 100 µg/ml in sterile PBS containing at least 0.1% human or bovine serum albumin.
기타 정보
Morvan, F. et al. 2006. J. Bone Miner. Res.21, 934.
Mukhopadhyay, M. et al. 2006. Development133, 2149.
Niehrs, C. 2006. Oncogene25, 7469.
Li, X. et al. 2005. Nat. Genet.37, 945.
Bullock, C.M. et al. 2004. Mol. Pharmacol.65, 582.
Mao, B. and C. Niehrs 2003. Gene302, 179.
Brott, B. and S.Y. Sokol 2002. Mol. Cell. Biol.22, 6100.
Li, L. et al. 2002. J. Biol. Chem.277, 5977.
Mao, B. et al. 2002. Nature417, 664.
Mao, B. et al. 2001. Nature411, 321.
Wu, W. et al. 2000. Current Biol.10,1611.
Monaghan, A.P. et al. 1999. Mech. Dev.87, 45.
Krupnik, V.E. et al. 1999. Gene238, 301.
Mukhopadhyay, M. et al. 2006. Development133, 2149.
Niehrs, C. 2006. Oncogene25, 7469.
Li, X. et al. 2005. Nat. Genet.37, 945.
Bullock, C.M. et al. 2004. Mol. Pharmacol.65, 582.
Mao, B. and C. Niehrs 2003. Gene302, 179.
Brott, B. and S.Y. Sokol 2002. Mol. Cell. Biol.22, 6100.
Li, L. et al. 2002. J. Biol. Chem.277, 5977.
Mao, B. et al. 2002. Nature417, 664.
Mao, B. et al. 2001. Nature411, 321.
Wu, W. et al. 2000. Current Biol.10,1611.
Monaghan, A.P. et al. 1999. Mech. Dev.87, 45.
Krupnik, V.E. et al. 1999. Gene238, 301.
법적 정보
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
11 - Combustible Solids
WGK
WGK 2
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
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