341583

FGF-2, Basic, Bovine

• 동의어(들): FGF-2, Basic, Bovine, nbbFGF, Fibroblast Growth Factor, Basic, Bovine
• CAS Number: 106096-93-9
• MDL number: MFCD00081382
• UNSPSC 코드: 12352202

속성

• 분석: ≥97% (SDS-PAGE)
• Quality Level: 100
• 형태: lyophilized
• 제조업체/상표: Calbiochem^®
• 저장 조건: OK to freeze
• 불순물: <0.1 ng/μg Endotoxin (ng/μg FGF)
• 배송 상태: ambient
• 저장 온도: −20°C

설명

일반 설명

Native, bovine basic fibroblast growth factor. A multi-functional factor with chemotactic activity for numerous cell types. A multifunctional factor with chemotactic activity for numerous cell types. Mitogenic to neuroectoderm- and mesoderm-derived cells. Plays a role in the proliferation of neuronal precursors and in neuronal differentiation. A potent angiogenic factor both in vivo and in vitro. Known to activate phopholipase D in endothelial cells. Higher concentrations are found in the brain and pituitary gland. Purified from bovine brain by sequential chromatography.

Native, bovine basic fibroblast growth factor. A multi-functional factor with chemotactic activity for numerous cell types. Mitogenic to neuroectoderm- and mesoderm-derived cells. Plays a role in the proliferation of neuronal precursors and in neuronal differentiation. A potent angiogenic factor both in vivo and in vitro. Activates phospholipase D in endothelial cells.

생화학적/생리학적 작용

ED₅₀ = 50-150 pg/ml, in the absence of heparin, for stimulation of ³H-thymidine incorporation by NR6R-3T3 fibroblasts

경고

Toxicity: Standard Handling (A)

물리적 형태

Lyophilized from sterile-filtered 20 mM Tris-HCl, 1M NaCl, 50 µg BSA/µg FGF, pH 7.0.

재구성

Following reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -70°C.

Reconstitute to a concentration of ≥10 µg/ml in sterile PBS containing ≥0.1% HSA or BSA.

기타 정보

Ohmiya, M., et al. 2001. J. Neurosci. Res.65, 228.
Murai, N., et al. 1996. J. Neurosurg. 85, 1072.
Thomas, K. 1987. Methods Enzymol.147, 120.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3