생물학적 소스
rabbit
Quality Level
항체 형태
serum
항체 생산 유형
primary antibodies
클론
polyclonal
형태
lyophilized
미포함
preservative
종 반응성
human, rat
제조업체/상표
Calbiochem®
저장 조건
OK to freeze
동형
IgG
배송 상태
ambient
저장 온도
2-8°C
타겟 번역 후 변형
unmodified
유전자 정보
human ... DRD4(1815)
rat ... Drd4(25432)
일반 설명
Anti-Dopamine D4 Receptor, rabbit polyclonal, recognizes ~48-51 kDa dopamine D4 receptor in rat brain. Does not cross-react with other dopamine receptors. It is validated for use in ELISA, WB & IHC.
Rabbit polyclonal antibody supplied as lyophilized, undiluted serum. Recognizes the ~48-51 kDa D4 dopamine receptor.
Recognizes the ~48-51 kDa dopamine D4 receptor in rat brain. Does not cross-react with other dopamine receptors.
면역원
Human
a synthetic peptide corresponding to amino acids 176-185 of human dopamine D₄ receptor
애플리케이션
ELISA (see comments)
Immunoblotting (1:500)
Immunohistochemistry (1:10,000)
Immunoblotting (1:500)
Immunohistochemistry (1:10,000)
경고
Toxicity: Standard Handling (A)
물리적 형태
Undiluted serum.
재구성
Reconstitute the lyophilized antibody with 100 µl PBS containing 10 mg/ml BSA. For further dilution of the antiserum use a similar buffer formulation. Following reconstitution, refrigerate (4°C). Variables associated with assay conditions will dictate the proper working dilution.
분석 메모
Positive Control
Rat brain
Rat brain
기타 정보
Mignini, F., et al. 2000. J. Autonom. Pharmacol.20, 325.
This antibody has also been reported to work for ELISA. For blocking immunoblot membranes, use ONLY 4% goat serum or evaporated goat′s milk. For immunohistochemistry, a 1:10,000 dilution of the reconstituted serum demonstrated specific staining of rat brain sections including olfactory bulb glomeruli, cortex layer 4, cortex layers 2 and 3 (dendrite staining), cortex layer 5 (apical dendrite staining), and the dentate gyrus of the hippocampus (diffuse staining in the molecular layer, punctuate staining in the granule cell layer).
법적 정보
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
11 - Combustible Solids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Alberto Ricci et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 50(8), 1091-1096 (2002-07-23)
Dopamine D4 receptors mediate inhibition of vasopressin-dependent sodium reabsorption by dopamine in collecting tubules. At present, the distribution of D4 receptors in other renal districts remains an open issue. The renal distribution of D4 receptor was assessed in normally innervated
Satoko Oda et al.
Frontiers in neuroanatomy, 17, 1111008-1111008 (2023-03-04)
Pial astrocytes, a cellular component of the cerebral cortex surface structure, are observed in a wide range of mammalian species. Despite being recognized as such, the functional potential of pial astrocytes has long been overlooked. Our previous research demonstrated that
Dana C Borcherding et al.
PloS one, 6(9), e25537-e25537 (2011-10-04)
Dopamine (DA) binds to five receptors (DAR), classified by their ability to increase (D1R-like) or decrease (D2R-like) cAMP. In humans, most DA circulates as dopamine sulfate (DA-S), which can be de-conjugated to bioactive DA by arylsulfatase A (ARSA). The objective
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