218705

Casein Kinase I Inhibitor, D4476

InSolution, ≥95%

• 동의어(들): InSolution Casein Kinase I Inhibitor, D4476
• 실험식(Hill 표기법): C₂₃H₁₈N₄O₃
• Molecular Weight: 398.41
• UNSPSC 코드: 12352200
• NACRES: NA.77

속성

• Quality Level: 100
• 분석: ≥95% (HPLC)
• 형태: liquid
• 제조업체/상표: Calbiochem^®
• 저장 조건: OK to freeze; desiccated (hygroscopic); protect from light
• 배송 상태: wet ice
• 저장 온도: −20°C

설명

일반 설명

A cell-permeable, potent, and relatively specific ATP-competitive inhibitor of CK1 (IC₅₀ = 200 nM from S. pombe; 0.3 µM for CK1δ) and ALK5 (IC₅₀ = 500 nM). A weak inhibitor of p38α MAP kinase (IC₅₀ = 12 µM) and several other kinases tested, including PKB, SGK, and GSK-3β. Suppresses the site-specific phosphorylation (Ser³²² and Ser³²⁵) and nuclear exclusion of FOXO1A in H4IIE hepatoma cells. Shown to be ~10-fold more potent than IC261 (Cat. No. 400090; IC₅₀ = 2.5 µM for CK1).

생화학적/생리학적 작용

Cell permeable: yes

Primary Target
CK1, CK1d

Product competes with ATP.

Reversible: no

Target IC₅₀: 0.2 µM, 0.3 µM against CK1, CK1d, respectively

포장

Packaged under inert gas

Please refer to vial label for lot-specific concentration.

경고

Toxicity: Irritant (B)

물리적 형태

A 10 mM (1 mg/251 µl) solution of Casein Kinase I Inhibitor, D4476 (Cat. No. 218696) in DMSO

재구성

Following initial use, aliquot and refrigerate (4°C).

기타 정보

Rena, G., et al. 2004. EMBO Reports5, 60.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany