207000

Calcineurin Autoinhibitory Peptide

The Calcineurin Autoinhibitory Peptide controls the biological activity of Mn2+-stimulated calcineurin activity. This small molecule/inhibitor is primarily used for Phosphorylation & Dephosphorylation applications.

• 동의어(들): Calcineurin Autoinhibitory Peptide, ITSFEEAKGLDRINERMPPRRDAMP
• 실험식(Hill 표기법): C₁₂₄H₂₀₅N₃₉O₃₉S₂
• Molecular Weight: 2930.32
• UNSPSC 코드: 12352202

속성

• Quality Level: 100
• 분석: ≥97% (HPLC)
• 형태: lyophilized
• 제조업체/상표: Calbiochem^®
• 저장 조건: OK to freeze; desiccated (hygroscopic)
• solubility: water: soluble
• 배송 상태: ambient
• 저장 온도: −20°C

설명

일반 설명

Corresponds to a C-terminal domain (residues 457-482) of the calmodulin-binding domain of calcineurin. Specific calcineurin inhibitor. Does not inhibit CaM kinase II, protein phosphatase I or 2A. Inhibits Mn²⁺-stimulated calcineurin activity (IC₅₀ = 10 µM using ³²P myosin light chain as a substrate) but has no effect on Ni²⁺-stimulated activity.

Specific calcineurin inhibitor. Corresponds to the C-terminal domain (residues 457-482) of the calmodulin-binding domain of calcineurin (PP2B). Inhibits Mn²⁺-stimulated calcineurin activity (IC₅₀ = 10 µM using ³²P-myosin light chain as a substrate) but has no effect on Ni²⁺-stimulated enzyme activity. Does not inhibit CaM kinase II, protein phosphatase 1 or 2A.

생화학적/생리학적 작용

Cell permeable: no

Primary Target
Mn²⁺-stimulated calcineurin activity

Product does not compete with ATP.

Reversible: no

Target IC₅₀: 10 µM using 32P-myosin light chain as a substrate

포장

Yes

경고

Toxicity: Standard Handling (A)

서열

H-Ile-Thr-Ser-Phe-Glu-Glu-Ala-Lys-Gly-Leu-Asp-Arg-Ile-Asn-Glu-Arg-Met-Pro-Pro-Arg-Arg-Asp-Ala-Met-Pro-OH

물리적 형태

Supplied as a trifluoroacetate salt.

재구성

Following reconstitution, aliquot and freeze (-20°C). Aqueous stock solutions are stable for up to 2 months at -20°C.

기타 정보

Perrino, B.A., et al. 1995. J. Biol. Chem. 270, 340.
Yokoyama, Y., and Wang, J.H. 1994. FEBS Lett.337, 128.
Yokoyama, N., et al. 1993. Arch. Biochem. Biophys.300, 615.
Matsui, H., et al. 1991. Biochem. Int.24, 1119.
Hashimoto, Y., et al. 1990. J. Biol. Chem.265, 1924.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable