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Merck
모든 사진(1)

문서

17-321

Sigma-Aldrich

Rap1 Activation Assay Kit

Non-radioactive Rap1 Activation Assay Kit that uses Ral GDS RBD, agarose (Catalog # 14-455) to precipitate Rap1-GTP from cell lysates & detection by a Rap1 specific antibody.

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모든 사진(1)

About This Item

UNSPSC 코드:
12161503
eCl@ss:
32161000
NACRES:
NA.84

Quality Level

100

제조업체/상표

Upstate®

기술

activity assay: suitable
affinity binding assay: suitable

NCBI 수납 번호

NM_021159.3

UniProt 수납 번호

P52306

배송 상태

dry ice

유전자 정보

human ... RAP1GDS1(5910)

포장

Kit capacity: 20-30 assays

성분

100X GTPγS, 10mM (Cat.# 20-176)

100X GDP, 100mM (Cat.# 20-177)

Rap1 Activation Lysis Buffer, 2X

Anti-Rap1

Rap1 Assay Reagent (Ral GDS-RBD, agarose) (Cat.# 14-455)

품질

Routinely evaluated by affinity precipitation assay. Ral GDS RBD, agarose precipitated GTP-Rap1 from HEK293 lysates. The precipitated GTP-Rap1 was detected by immunoblot analysis using Anti-Rap1.

법적 정보

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

픽토그램

Exclamation mark
GHS07

신호어

Warning

유해 및 위험 성명서

H319

Hazard Classifications

Eye Irrit. 2

Storage Class Code

10 - Combustible liquids

시험 성적서(COA)

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문서 라이브러리 방문

Uma Potla et al.
The Journal of clinical investigation, 124(4), 1757-1769 (2014-03-20)
Injury to the specialized epithelial cells of the glomerulus (podocytes) underlies the pathogenesis of all forms of proteinuric kidney disease; however, the specific genetic changes that mediate podocyte dysfunction after injury are not fully understood. Here, we performed a large-scale
Measurement of GTP-bound Ras-like GTPases by activation-specific probes.
M van Triest et al.
Methods in enzymology, 333, 343-348 (2001-06-13)
D Sakkab et al.
The Journal of biological chemistry, 275(15), 10772-10778 (2001-02-07)
Hepatocyte growth factor (HGF; scatter factor) is a multipotent protein with mitogenic, motogenic, and developmental functions. Upon activation, the HGF-receptor c-Met binds and phosphorylates the multisite docking protein Gab1. Besides binding motifs for phosphatidylinositol 3-kinase and Grb2, Gab 1 contains
Emilie Montenont et al.
Blood advances, 5(9), 2362-2374 (2021-05-05)
Human anucleate platelets cannot be directly modified using traditional genetic approaches. Instead, studies of platelet gene function depend on alternative models. Megakaryocytes (the nucleated precursor to platelets) are the nearest cell to platelets in origin, structure, and function. However, achieving
Rasmus Koefoed Petersen et al.
Molecular and cellular biology, 28(11), 3804-3816 (2008-04-09)
Cyclic AMP (cAMP)-dependent processes are pivotal during the early stages of adipocyte differentiation. We show that exchange protein directly activated by cAMP (Epac), which functions as a guanine nucleotide exchange factor for the Ras-like GTPases Rap1 and Rap2, was required
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