16-318

Anti-Akt/PKB Antibody, PH Domain, clone SKB1, Magnetic Bead Conjugate

clone SKB1, from mouse

• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: SKB1, monoclonal
• 종 반응성: mouse, rat, human
• 기술: immunoprecipitation (IP): suitable
• 동형: IgG
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: mouse ... Akt1(11651)

설명

일반 설명

Akt (protein kinase B), a serine/threonine kinase, has emerged as a critical enzyme in signal transduction pathways involved in cell proliferation, apoptosis, angiogenesis, and diabetes. In mammals three isoforms of Akt (α, β, γ or Akt 1, 2, 3) are reported that exhibit a high degree of homology, but differ slightly in the localization of their regulatory phosphorylation sites. Akt α is the predominant isoform in most tissues, whereas the highest expression of Akt β is observed in the insulin-responsive tissues, and Akt γ is abundant in brain tissue. Each Akt isoform is composed of three functionally distinct regions: an N-terminal pleckstrin homology (PH) domain that provides a lipid-binding module to direct Akt to PIP2 and PIP3, a central catalytic domain, and a C-terminal hydrophobic motif.

특이성

Recognizes Akt1/PKBα.

면역원

GST-fusion protein corresponding to residues 1-149 of human Akt1

애플리케이션

Detect Akt/PKB using this Anti-Akt/PKB Antibody, PH Domain, clone SKB1, Magnetic Bead Conjugate validated for use in IP.

Research Category
Signaling

Research Sub Category
PI3K, Akt, & mTOR Signaling

품질

Evaluated by immunoprecipitation on 250 μg of NIH3T3 whole cell lysate stimulated with 50 ng/mL PDGF (5 minutes).

표적 설명

~ 60 kDa observed

물리적 형태

20% slurry; 1X PBS, 0.05% sodium azide.
Sufficient for 10 IPs.

Format: Purified

Protein G Chromatography

저장 및 안정성

Stable for 1 year at 2-8ºC from date of receipt.

분석 메모

Control
NIH3T3 whole cell lysate stimulated with 50 ng/mL PDGF (5 minutes)

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2