14-752-M

GRK7 Protein, active, 10 µg

• UNSPSC 코드: 12352202
• eCl@ss: 32160405
• NACRES: NA.32

속성

• 생물학적 소스: human
• Quality Level: 100; 300
• 분자량: M_w 66 kDa
• 제조업체/상표: Upstate^®
• 기술: activity assay: suitable (kinase)
• NCBI 수납 번호: NM_139209.2
• UniProt 수납 번호: Q8WTQ7

설명

일반 설명

N-terminal 6His-tagged, recombinant, full length human GRK7 expressed by baculovirus in Sf21 cells.

Recombinant, full length human GRK7 expressed by baculovirus in Sf21 cells.

애플리케이션

Active, N-terminal 6His-tagged, recombinant, full length human GRK7 expressed by baculovirus in Sf21 cells. For use in Kinase Assays.

Research Category
Metabolism

Research Sub Category
Cardiovascular Disease|Metabolic Disorders

생화학적/생리학적 작용

• Protein Target: GRK7
• Target Sub-Family:AGC

품질

Routinely evaluated by phosphorylation of 2 mg/mL casein.
Purity was assessed by SDS-PAGE and Coomassie blue staining using 3μg of GRK7.

단위 정의

Specific Activity: where one unit of GRK7 activity is defined as 1nmol phosphate incorporated into 2mg/ml casein per minute at 30°C with a final ATP concentration of 100μM.

물리적 형태

10μg of recombinant protein in 100 μl of 50 mM Tris-HCl pH 8.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.

Format: Purified using Ni²⁺/NTA agarose. Purity 99% by SDS-PAGE and Coomassie blue staining.

저장 및 안정성

Stable for 6 months at -70°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.

법적 정보

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial

안전성 정보

• 픽토그램: GHS07
• 신호어: Warning
• 유해 및 위험 성명서: H317
• 예방조치 성명서: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1
• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable