14-750-M

Abl (H396P) Protein, active, 10 µg

Active, N-terminal 6His-tagged recombinant human Abl, residues 27-end containing the H396P mutation. Expressed by baculovirus in Sf21 insect cells. For use in Kinase Assays.

• UNSPSC 코드: 12352202
• eCl@ss: 32160405
• NACRES: NA.32

속성

• 생물학적 소스: human
• Quality Level: 300
• 재조합: expressed in baculovirus infected Sf21 cells
• 분자량: M_w 121 kDa
• 제조업체/상표: Upstate^®
• 기술: activity assay: suitable (kinase)
• UniProt 수납 번호: P00519; U07563

설명

일반 설명

Product Source: N-terminal 6His-tagged recombinant human Abl, residues 27-end containing the H396P mutation. Expressed by baculovirus in Sf21 insect cells.

The Abl tyrosine kinase inhibitor STI571 (Gleevec) is an effective therapy for stable-phase chronic myeloid leukemia patients. Many patients responding to STI571 later relapse, due to a reactivation of Bcr-Abl activity. In certain cases this appears to correlate with the presence of the H396P mutation, which confers resistance to drug binding due to steric interference. (Corbin AS. et al, Blood, (2002); 101:4611-4614 and La Rosee P. et al, Cancer Research; 62:7149-7153).

애플리케이션

Research Category
Metabolism

Research Sub Category
Cardiovascular Disease

Metabolic Disorders

생화학적/생리학적 작용

Protein Target: Abl

Target Sub-Family: TK

포장

Also available in 250μg size as catalog # 14-750M - Call for pricing and availability.

품질

Routinely evaluated by phosphorylation of Abltide.

물리적 형태

50mM Tris/HCl pH7.5, 150mM NaCl, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1mM EGTA, 0.1% 2-mercaptoethanol, 0.03% Brij 35. Frozen solution.

Ni²⁺/NTA-agarose

저장 및 안정성

Store -70°C. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.

기타 정보

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

법적 정보

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• 픽토그램: GHS07
• 신호어: Warning
• 유해 및 위험 성명서: H317
• 예방조치 성명서: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1
• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable