14-745-M

TAO3 Protein, active, 10 µg

Active, N-terminal 6His-tagged, recombinant, human TAO3 amino acids 1-411, N47S. For use in Kinase Assays.

• UNSPSC 코드: 12352202
• eCl@ss: 32160405
• NACRES: NA.32

속성

• 생물학적 소스: human
• Quality Level: 100
• 재조합: expressed in baculovirus infected Sf21 cells
• 분석: 84% (Coomassie blue staining, SDS-PAGE)
• 특이 활성도: 915 U/mg
• 분자량: M_w 50.2 kDa
• 제조업체/상표: Upstate^®
• 기술: activity assay: suitable (kinase)
• NCBI 수납 번호: NM_016281.3
• UniProt 수납 번호: Q9H2K8
• 유전자 정보: human ... TAOK3(51347)

설명

일반 설명

N-terminal 6His-tagged, recombinant, human TAO3 amino acids 1-411; N47S.

Product Source: Recombinant, human TAO3 amino acids 1-411; N47S. Expressed by baculovirus in Sf21 cells.

애플리케이션

Research Category
Metabolism

Research Sub Category
Cardiovascular Disease

Metabolic Disorders

생화학적/생리학적 작용

Protein Target: TAO3

Target Sub-Family: STE

품질

Routinely evaluated by phosphorylation of 0.8 mg/mL myelin basic protein.
Purity was assessed by SDS-PAGE and Coomassie blue staining using 3μg of TAO3.

단위 정의

Specific Activity: where one unit of TAO3 activity is defined as 1nmol phosphate incorporated into 0.8mg/ml myelin basic protein per minute at 30°C with a final ATP concentration of 100μM.

물리적 형태

10 μg of enzyme in 6.3 μL of 50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij 35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.

Ni²⁺/NTA-agarose

저장 및 안정성

Stable for 6 months at -70°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.

법적 정보

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.