13-104

MBP (myelin basic protein), bovine, purified

• UNSPSC 코드: 12352202
• eCl@ss: 32160405
• NACRES: NA.41

속성

• 생물학적 소스: bovine
• Quality Level: 100
• 형태: liquid
• 유통기한: 2 yr
• 제조업체/상표: Upstate^®
• 기술: activity assay: suitable
• solubility: water: soluble
• NCBI 수납 번호: NM_001025081.1; NM_001025090.1; NM_001025092.1; NM_001025094.1; NM_001025098.1; NM_001025100.1; NM_001025101.1; NM_002385.2
• UniProt 수납 번호: P02686
• 배송 상태: wet ice
• 저장 온도: −20°C
• 유전자 정보: bovine ... MBP(618684)

설명

일반 설명

Myelin basic protein (MBP) belongs to the intrinsically disordered protein family. It is localized in the insulating layer of nerve cells in the brain and spinal cord. It is an abundant protein found in the central nervous system (CNS).

Research area: Cell Signaling
MBP is an alkaline protein with a pI of 11.6, constituting approximately 30% of the total protein content of the myelin in the central nervous system.

애플리케이션

MBP (myelin basic protein), bovine, purified has been used in western blotting and as a standard in enzyme-linked immunosorbent assay (ELISA).

생화학적/생리학적 작용

MBP can interact with various polyanionic proteins such as actin, tubulin, Ca²⁺-calmodulin, and clathrin, as well as negatively charged lipids. Upon binding to these proteins, MBP undergoes structural changes. It is believed to function as a membrane actin-binding protein, potentially enabling its involvement in relaying extracellular signals to the cytoskeleton in oligodendrocytes and tight junctions in myelin. External signals received by myelin or cultured oligodendrocytes lead to alterations in the phosphorylation of MBP, indicating its participation in signaling. The peptide MBP4-14 is specific for protein kinase C and is resistant to phosphatase activity. Moreover, MBP is extensively utilized in the "in gel" kinase assay, making it a vital biochemical for studying protein kinase activities.

Myelin basic protein (MBP), known as the ‘executive molecule of myelin′, helps in the formation of central nervous system (CNS) myelin. Elevated levels of MBP in the cerebrospinal fluid (CSF) are detected in acute neurological disorders like exacerbating multiple sclerosis, and traumatic brain injury. It is also observed at increased levels during chronic progressive disorders including dementia, tumor cerebri, and metabolic disorders. 

품질

routinely evaluated by successfully phosphorylating MAP Kinase 2/Erk2, active (Catalog # 14-173)

물리적 형태

10mM MOPS, pH 7.0, with 0.05% sodium azide

저장 및 안정성

2 years at -20°C

기타 정보

This product is derived from bovine source. Export of this product to certain countries may be restricted. Please contact Customer Service or your local distributor to inquire about product availability and export options.

법적 정보

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable