1.15943

Methylene blue (C.I.52015)

for microscopy Certistain^®

• 동의어(들): Methylene Blue trihydrate, Methylthioninium chloride, 3,7-Bis(dimethylamino)phenothiazinium chloride, Tetramethylthionine chloride
• 실험식(Hill 표기법): C₁₆H₁₈ClN₃S · 3H₂O
• CAS Number: 7220-79-3
• Molecular Weight: 373.90
• Beilstein: 3599847
• MDL number: MFCD00012111
• UNSPSC 코드: 41116130
• EC 인덱스 번호: 200-515-2

속성

• Quality Level: 400
• 형태: solid
• 효능: 1180 mg/kg LD₅₀, oral (Rat)
• 구성: Dye, ≥82% spectrophotometric assay
• pH: 3 (20 °C, 10 g/L in H₂O)
• mp: 190 °C (dec.) (lit.)
• solubility: 50 g/L
• density: 1.757 g/cm³ at 22 °C
• 벌크 밀도: 400‑600 kg/m³
• εmax: 2250-2750 at 660-665 nm in 50% ethanol at 0.003 g/L
• 응용 분야: diagnostic assay manufacturing; hematology; histology
• 저장 온도: 2-30°C
• SMILES string: [Cl-].CN(C)c1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C
• InChI: 1S/C16H18N3S.ClH/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;/h5-10H,1-4H3;1H/q+1;/p-1
• InChI key: CXKWCBBOMKCUKX-UHFFFAOYSA-M

설명

일반 설명

Methylene blue (C.I. 52015) for microscopy Certistain^® is a dry dye that is used in bacteriology for an overview stain and as counterstaining for Ziehl-Neelsen method in sample material of human origin. Bacteria appear blue. The dye has a further important application in hematology: Methylene blue and eosin are the main components of many staining solutions and dye mixtures. Certistain^® is the product group for seleceted high quality dyes that fulfill all quality requirements of IVD and CE registration. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

분석 메모

Dye content (spectrophotometrically): ≥ 82 %
Identity
- Chloride:
passes test
- UV/VIS-Spectrum: passes test
Absorption maximum λ max (ethanol 50 %): 660 - 665 nm
Spec. Absorptivity A 1%/1cm (λ_max; 0.003 g/l; ethanol 50 %): 2250 - 2750
TLC-Test: passes test
Loss on drying (110 °C): 10 - 15 %
Suitability for microscopy: passes test

법적 정보

CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• 픽토그램: GHS07
• 신호어: Warning
• 유해 및 위험 성명서: H302
• 예방조치 성명서: P264 - P270 - P301 + P312 - P501
• Hazard Classifications: Acute Tox. 4 Oral
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable