추천 제품
생물학적 소스
rabbit
Quality Level
항체 형태
affinity isolated antibody
항체 생산 유형
primary antibodies
클론
polyclonal
정제법
affinity chromatography
종 반응성
human
제조업체/상표
Upstate®
기술
western blot: suitable
동형
IgG
NCBI 수납 번호
배송 상태
dry ice
타겟 번역 후 변형
phosphorylation (pTyr507/pThr508)
유전자 정보
human ... LIMK1(3984)
특이성
Predicted to cross-react with Mouse, Rat, and Frog (100% homologous) based on sequence homology.
Recognizes phosphorylated LIMK1/2 (Tyr507/Thr508)
면역원
Peptide corresponding to amino acid region encompassing the human, mouse, rat and chicken phospho-LIMK1/2 (Tyr507/Thr508)
애플리케이션
Detect phospho-LIMK 1/2 (Tyr507/Thr508) using this anti-phospho-LIMK 1/2 (Tyr507/Thr508) Antibody validated for use in WB.
Immunoblot Analysis: A 1:1000 dilution of this lot detected phosphorylated LIMK 1/2 (Tyr507/Thr508) in RIPA lysates of SDF-1α treated Jurkat cells.
Research Category
Signaling
Signaling
Research Sub Category
Cytoskeletal Signaling
Cytoskeletal Signaling
품질
Routinely evaluated by immunoblot.
표적 설명
72 kDa (LIMK1) and 65 kDa (LIMK2)
물리적 형태
100 μL of affinity purified rabbit polyclonal IgG in 50% storage buffer (PBS (without Mg2+ and Ca2+), pH 7.3 containing 1.0 mg/mL BSA (IgG, protease free) and 0.05% sodium azide) and 50% glycerol.
ImmunoAffinity Purified
저장 및 안정성
Stable for 2 years at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
법적 정보
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Activation of LIM-kinase by Pak1 couples Rac/Cdc42 GTPase signalling to actin cytoskeletal dynamics.
D C Edwards et al.
Nature cell biology, 1(5), 253-259 (1999-11-13)
Extracellular signals regulate actin dynamics through small GTPases of the Rho/Rac/Cdc42 (p21) family. Here we show that p21-activated kinase (Pak1) phosphorylates LIM-kinase at threonine residue 508 within LIM-kinase's activation loop, and increases LIM-kinase-mediated phosphorylation of the actin-regulatory protein cofilin tenfold
Down-regulation of PKCzeta expression inhibits chemotaxis signal transduction in human lung cancer cells.
Ying Liu,Bing Wang,Jingna Wang,Wuzhou Wan,Ronghua Sun,Yuliang Zhao,Ning Zhang
Lung Cancer null
Juliana Soosairajah et al.
The EMBO journal, 24(3), 473-486 (2005-01-22)
Slingshot (SSH) phosphatases and LIM kinases (LIMK) regulate actin dynamics via a reversible phosphorylation (inactivation) of serine 3 in actin-depolymerizing factor (ADF) and cofilin. Here we demonstrate that a multi-protein complex consisting of SSH-1L, LIMK1, actin, and the scaffolding protein
Hirobumi Tada et al.
Proceedings of the National Academy of Sciences of the United States of America, 113(45), E7097-E7105 (2016-10-30)
Social separation early in life can lead to the development of impaired interpersonal relationships and profound social disorders. However, the underlying cellular and molecular mechanisms involved are largely unknown. Here, we found that isolation of neonatal rats induced glucocorticoid-dependent social
K Ohashi et al.
The Journal of biological chemistry, 275(5), 3577-3582 (2000-02-01)
LIM-kinase 1 (LIMK1) phosphorylates cofilin, an actin-depolymerizing factor, and regulates actin cytoskeletal reorganization. LIMK1 is activated by the small GTPase Rho and its downstream protein kinase ROCK. We now report the site of phosphorylation of LIMK1 by ROCK. In vitro
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