07-448-S

Anti-monomethyl-Histone H3 (Lys27) Antibody, Trial Size

Upstate^®, from rabbit

• 동의어(들): H3K27me1, Histone H3 (monomethyl K27), H3 histone family, member T, Histone 3, H3, Histone cluster 3, H3
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 종 반응성: human, vertebrates
• 제조업체/상표: Upstate^®
• 기술: ChIP: suitable (ChIP-seq); dot blot: suitable; immunocytochemistry: suitable; western blot: suitable
• 동형: IgG
• NCBI 수납 번호: NP_003484
• UniProt 수납 번호: Q16695
• 배송 상태: wet ice
• 타겟 번역 후 변형: monomethylation (Lys27)
• 유전자 정보: human ... HIST1H3F(8968)

설명

일반 설명

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

특이성

Broad species cross-reactivity expected, based on sequence homology.

Recognizes monomethylated histone H3 (Lys27), Mr 17 kDa.

면역원

Epitope: Lys27

KLH-conjugated, synthetic 2X-branched peptide containing the sequence …ARmeKSA… in which meK corresponds to monomethyl-lysine at residue 27 of human histone H3.

애플리케이션

Anti-monomethyl-Histone H3 (Lys27), Trial Size Antibody is a Rabbit Polyclonal Antibody for detection of Histone H3 monomethylated on lysine 27 (H3K27me1). This antibody has dot blot (DB) verified specificity and is validated in, ICC, WB, ChIP, ChIP-seq. Small pack size is ideal for screening.

Immunocytochemistry & Dot Blot:
Reported by an independent laboratory (Kohlmaier, A., 2004; Perez-Burgos, L., 2004; Peters, A. H., 2003).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

품질

Routinely evaluated by western blot acid in extracted proteins from Hela cells.

Western Blot Analysis:
0.1-0.2 μg/mL had detected monomethylated histone H3 in acid extracted proteins from HeLa cells.

표적 설명

17 kDa

물리적 형태

Format: Purified

Protein A purified

Purified rabbit IgG in buffer containing 0.01 M phosphate buffer, pH 7.6, 0.125 M NaCl, 0.05% sodium azide and 30% glycerol.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

분석 메모

Control
HeLa acid extract

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

법적 정보

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable