07-2276-I

Anti-mSin1 Antibody

from rabbit, purified by affinity chromatography

• 동의어(들): Target of rapamycin complex 2 subunit MAPKAP1, TORC2 subunit MAPKAP1, Mitogen-activated protein kinase 2-associated protein 1, Stress-activated map kinase-interacting protein 1, SAPK-interacting protein 1, mSIN1, mSin1
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 정제법: affinity chromatography
• 종 반응성: rat, human, mouse
• 종 반응성(상동성에 의해 예측): porcine (based on 100% sequence homology), feline (based on 100% sequence homology), horse (based on 100% sequence homology), hamster (based on 100% sequence homology), bovine (based on 100% sequence homology)
• 기술: immunoprecipitation (IP): suitable; western blot: suitable
• NCBI 수납 번호: NP_001006618
• UniProt 수납 번호: Q9BPZ7
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... MAPKAP1(79109)

설명

일반 설명

Target of rapamycin complex 2 subunit MAPKAP1 (UniProt Q9BPZ7; also known as MEKK2-interacting protein 1, Mitogen-activated protein kinase 2-associated protein 1, mSIN1, Ras inhibitor MGC2745, SAPK-interacting protein 1, Stress-activated protein kinase-interacting 1, Stress-activated map kinase-interacting protein 1,, TORC2 subunit MAPKAP1) is encoded by the MAPKAP1 (also known as JC310, MIP1 SIN1, SIN1b, SIN1g) gene (Gene ID 79109) in human. mSIN1 is an essential component of the Torc2 complex. mTORC2 is comprised of mTOR, a large Ser/Thr protein kinase, along with mSIN1, GbL (mLST8), Protor1, Protor2, and Rictor. The mTORC2 complex is activated primarily downstream of PI3 Kinase and is known to affect cell proliferation and survival primarily by phosphorylating Akt on Ser473. Additionally, the mTORC2 complex is known to affect cytoskeletal organization and cell migration by exerting its effects through Rac, Rho, and PKC. mSIN1 is also known to directly interact with Ras, SPAKs, and JNK.

특이성

Expected to react with spliced isoforms 1, 2, 3, 5, 6, but not 4.

면역원

Epitope: MAP3K2/NBN interaction domain

KLH-conjugated linear peptide corresponding to the MAP3K2/NBN interaction domain of human mSin1.

애플리케이션

Detect MAPKAP1/Sin1 using this rabbit polyclonal Anti-mSin1 Antibody, Cat. No. 07-2276-I, validated for use in Western Blotting and Immunoprecipitation.

Immunoprecipitation Analysis: 5 µg from a representative lot immunoprecipitated mSin1 in 0.5 mg of A431 cell lysate.

Research Category
Signaling

Research Sub Category
Signaling Neuroscience

품질

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected mSin1 in 10 µg of A431 cell lysate.

표적 설명

~59 kDa observed

물리적 형태

Affinity purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

기타 정보

Concentration: Please refer to lot specific datasheet.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable