추천 제품
생물학적 소스
rabbit
Quality Level
항체 형태
affinity isolated antibody
항체 생산 유형
primary antibodies
클론
polyclonal
정제법
affinity chromatography
종 반응성
vertebrates, Tetrahymena sp., Xenopus, human
기술
multiplexing: suitable
western blot: suitable
NCBI 수납 번호
UniProt 수납 번호
배송 상태
wet ice
타겟 번역 후 변형
dimethylation (Arg26)
유전자 정보
human ... H3C1(8350)
일반 설명
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
특이성
Recognizes CARM1 in vitro methylated recombinant Histone H3. The immunizing sequence is conserved from Tetrahymena through human therefore a broad cross reactivity is expected.
Recognizes dimethylated Histone H3 at and around arginine 26, MW ~17 kDa. Possible slight reactivity with monomethyl histone H3 (Arg26).
면역원
Epitope: Dimethylated Histone H3 (Arg26)
KLH-conjugated synthetic peptide (ATKAA(di-Me)RKSAPA-C) corresponding to amino acids 21-31 of Human Histone H3.
애플리케이션
Anti-dimethyl-Histone H3 (Arg26) Antibody is a rabbit polyclonal antibody for detection of dimethyl-Histone H3 (Arg26) also known as H3R26me2, Histone H3 (di methyl R26) & has been validated in WB, Mplex.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
SNAP I.D. (Western Blotting Technique):
A previous lot of this antibody worked successfully using the SNAP I.D. western blotting device and technique.
A previous lot of this antibody worked successfully using the SNAP I.D. western blotting device and technique.
품질
Evaluated by western blot on untreated and CARM1 in vitro methylated recombinant Histone H3.
Western Blot Analysis: 1:8000 dilution of this antibody detected dimethyl-Histone H3 (Arg26) in untreated and CARM1 in vitro methylated recombinant Histone H3.
Western Blot Analysis: 1:8000 dilution of this antibody detected dimethyl-Histone H3 (Arg26) in untreated and CARM1 in vitro methylated recombinant Histone H3.
표적 설명
17kDa
물리적 형태
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4) 150 mM NaCl, with 0.05% sodium azide.
저장 및 안정성
Stable for 1 year at 2-8ºC from date of receipt.
분석 메모
Control
CARM1 in vitro methylated recombinant Histone H3.
CARM1 in vitro methylated recombinant Histone H3.
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
적합한 제품을 찾을 수 없으신가요?
당사의 제품 선택기 도구.을(를) 시도해 보세요.
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Monica Mann et al.
Carcinogenesis, 34(7), 1468-1475 (2013-03-15)
Estrogen receptor alpha (ERα) is implicated in the initiation and progression of breast cancer and its transcription depends on the modulation of epigenetic changes at target gene promoters via coregulators. There is a critical need to understand the molecular mechanism(s)
Adam Burton et al.
Cell reports, 5(3), 687-701 (2013-11-05)
Cell plasticity or potency is necessary for the formation of multiple cell types. The mechanisms underlying this plasticity are largely unknown. Preimplantation mouse embryos undergo drastic changes in cellular potency, starting with the totipotent zygote through to the formation of
B Ruthrotha Selvi et al.
The Journal of biological chemistry, 285(10), 7143-7152 (2009-12-22)
Methylation of the arginine residues of histones by methyltransferases has important consequences for chromatin structure and gene regulation; however, the molecular mechanism(s) of methyltransferase regulation is still unclear, as is the biological significance of methylation at particular arginine residues. Here
Donghang Cheng et al.
The Journal of biological chemistry, 295(50), 17060-17070 (2020-10-04)
CARM1 is a protein arginine methyltransferase (PRMT) that acts as a coactivator in a number of transcriptional programs. CARM1 orchestrates this coactivator activity in part by depositing the H3R17me2a histone mark in the vicinity of gene promoters that it regulates.
Lifang Niu et al.
Plant physiology, 148(1), 490-503 (2008-07-29)
CARM1/PRMT4 (for COACTIVATOR-ASSOCIATED ARGININE METHYLTRANSFERASE1/PROTEIN ARGININE METHYLTRANSFERASE4) catalyzes asymmetric dimethylation on arginine (Arg), and its functions in gene regulation is understood only in animal systems. Here, we describe AtPRMT4a and AtPRMT4b as a pair of Arabidopsis (Arabidopsis thaliana) homologs of
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.