07-1229

Anti-DAPK2 Antibody

from rabbit, purified by affinity chromatography

• 동의어(들): DAP kinase, DAP- kinase-related protein, Death-associated protein kinase
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: affinity purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 정제법: affinity chromatography
• 종 반응성: rat, mouse, human
• 기술: immunohistochemistry: suitable (paraffin); western blot: suitable
• 동형: IgG
• NCBI 수납 번호: NM_014326
• UniProt 수납 번호: Q9UIK4
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... DAPK2(23604) ; rat ... Dapk2(300799)

설명

일반 설명

Death Associated Protein Kinase (DAPK) is a Ca2+/Calmodulin-dependent, cytoskeletal associated protein kinase whose expression is implicated in the sensitivity of cells to apoptotic effects of TNFα and Interferon-γ. Deletion of the Calmodulin-binding domain results in a constitutively active mutant with a more potent cytotoxic effect. In contrast, the catalytically inactive mutant exerts a dominant negative effect, reducing cytotoxicity and protecting cells from Interferon-γ induced cell death. DAPK expression is frequently lost in human carcinomas and B-cell leukemia, and lower levels of expression correlate with high rates of metastasis.

특이성

Detects a 42 kDa band by western blot, corresponding to DAPK2. Does not cross-react with DAPK1.

면역원

Peptide corresponding to aa 356-370 (ARRKALHPRRRSSTS) of human DAPK2, which is identical to the sequence of mouse origin.

애플리케이션

Detect DAPK2 using this Anti-DAPK2 Antibody validated for use in WB, IH(P).

Immunohistochemisty (paraffin): DAPK2 representative staining pattern/morphology in apoptotic bodies in colon cancer. Tissue was pretreated with citrate pH 6, antigen retrieval. A previous lot of this antibody was diluted to 1:100, using IHC-Select Detection with HRP-DAB.

Western Blot: A previous lot of this antibody was tested on multiple lysates. See datasheet.

Research Category
Signaling

Research Sub Category
GPCR, cAMP/cGMP & Calcium Signaling

품질

Routinely evaluated by Western Blot on C2C12 lysates.

Western Blot Analysis:
1:500-1,1000 dilution of this lot detected DAPK2 on 10 μg of C2C12 lysates.

표적 설명

Detects a 42 kDa band by western blot.

결합

Replaces: AB3606

물리적 형태

Affinity purified

Purified rabbit polyclonal IgG in buffer containing PBS and 0.02% sodium azide.

저장 및 안정성

Stable for 6 months at 2-8° C from date of receiept.

분석 메모

Control
C2C12 cell lysate

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable