05-637-AF488

Anti-Bmi-1 Antibody, clone F6, Alexa Fluor^™ 488

clone F6, from mouse, ALEXA FLUOR^™ 488

• 동의어(들): Polycomb complex protein BMI-1, Polycomb group RING finger protein 4, RING finger protein 51, Bmi-1
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 결합: ALEXA FLUOR^™ 488
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: F6, monoclonal
• 종 반응성: human, monkey
• 종 반응성(상동성에 의해 예측): rabbit (based on 100% sequence homology), mouse (based on 100% sequence homology), rat (based on 100% sequence homology)
• 기술: immunocytochemistry: suitable
• 동형: IgG1
• NCBI 수납 번호: NP_031578.2
• UniProt 수납 번호: P25916
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... BMI1(648)

설명

일반 설명

Polycomb complex protein BMI-1 (UniProt P25916; also known as B lymphoma Mo-MLV insertion region 1, Polycomb group RING finger protein 4) is encoded by the Bmi1 (also known as AW546694, Bmi-1, Pcgf4) gene (Gene ID 12151) in murine species. Bmi1 is a member of the polycomb group (PcG) family of evolutionarily conserved gene silencers involved in cell fate decisions and body pattern during development. Bmi1 is an important regulator of self-renewal of hematopoietic and neural stem cells, as well as cancer stem cells (CSCs). Bmi1 is a main constituent of the polycomb repressive complex 1 (PRC1) and pariticipates in PRC-mediated gene silencing by promoting monoubiquitination of H2A. Bmi1 overexpression is reported in many different human cancers and high level of Bmi1 is considered as a poor prognostic marker. Bmi1 targets genes involved in transforming growth factor-β/bone morphogenetic protein (TGF-beta/BMP), endoplasmic reticulum (ER) stress, and WNT pathways. Bmi itself is transcriptionally regulated by c-Myc and E2F1 transcription factors.

면역원

Epitope: a.a. 1-202

Recombinant Bmi-1 protein corresponding to residues 1-202 of mouse Bmi-1.

애플리케이션

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Nuclear Receptors

The unconjugated version (Cat. No. 05-637) is also shown to be suitable for Western blotting, Immunocytochemistry, and Immunohistochemistry applications.

This Anti-Bmi-1 Antibody, clone F6, Alexa Fluor^™ 488 is validated for use in Immunocytochemistry for the detection of Bmi-1.

품질

Evaluated by Immunocytochemistry in HepG2 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Bmi-1 in HepG2 cells.

표적 설명

40-44 kDa observed

물리적 형태

Protein G Purified

Purified mouse conjugate antibody in PBS with 15 mg/ml BSA and 0.1% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

기타 정보

Concentration: Please refer to lot specific datasheet.

법적 정보

ALEXA FLUOR is a trademark of Life Technologies

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable