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Merck
모든 사진(1)

주요 문서

05-312

Sigma-Aldrich

Anti-E2F-4 Antibody, clone GG22-2A6

clone GG22-2A6, Upstate®, from mouse

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About This Item

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41

생물학적 소스

mouse

Quality Level

항체 형태

purified immunoglobulin

항체 생산 유형

primary antibodies

클론

GG22-2A6, monoclonal

종 반응성

rat, mouse, human

제조업체/상표

Upstate®

기술

ChIP: suitable (ChIP-seq)
immunoprecipitation (IP): suitable
western blot: suitable

동형

IgG2b

NCBI 수납 번호

UniProt 수납 번호

배송 상태

dry ice

타겟 번역 후 변형

unmodified

유전자 정보

human ... E2F4(1874)

특이성

E2F-4; does not recognize E2F-1, -2, -3, or -5

면역원

GST fusion to human E2F-4

애플리케이션

Detect E2F-4 with Anti-E2F-4 Antibody, clone GG22-2A6 (Mouse Monoclonal Antibody), that has been shown to work in IP & WB.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors

품질

routinely evaluated on non-stimulated A431 cell lysate

표적 설명

55kDa

물리적 형태

200μg of protein G purified mouse IgG2b in 200μl of 0.07 M Tris-glycine, pH 7.4, 0.135 M NaCl, 0.035% sodium azide and 30% glycerol.
Format: Purified
Protein G Chromatography

저장 및 안정성

1 year at -20°C

분석 메모

Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.

법적 정보

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1


시험 성적서(COA)

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Dynamic loss of H2B ubiquitylation without corresponding changes in H3K4 trimethylation during myogenic differentiation.
Vethantham, V; Yang, Y; Bowman, C; Asp, P; Lee, JH; Skalnik, DG; Dynlacht, BD
Molecular and cellular biology null
Functional interaction between E2F-4 and p130: evidence for distinct mechanisms underlying growth suppression by different retinoblastoma protein family members.
Vairo, G, et al.
Genes & Development, 9, 869-881 (1995)
Erawan Borkham-Kamphorst et al.
Journal of cell communication and signaling, 6(1), 11-25 (2011-07-13)
Nephroblastoma overexpressed gene encodes a matricellular protein (CCN3/NOV) of the CCN family, comprising CCN1 (CYR61), CCN2 (CTGF), CCN4 (WISP-1), CCN5 (WISP-2), and CCN6 (WISP-3). CCN proteins are involved in the regulation of mitosis, adhesion, apoptosis, extracellular matrix production, growth arrest
Jiahui Du et al.
Development (Cambridge, England), 148(8) (2021-03-27)
Stem cells self-renew or give rise to transit-amplifying cells (TACs) that differentiate into specific functional cell types. The fate determination of stem cells to TACs and their transition to fully differentiated progeny is precisely regulated to maintain tissue homeostasis. Arid1a
L Wu et al.
Molecular and cellular biology, 20(19), 7059-7067 (2000-09-13)
Expression of the bovine papillomavirus E2 protein in cervical carcinoma cells represses expression of integrated human papillomavirus (HPV) E6/E7 oncogenes, followed by repression of the cdc25A gene and other cellular genes required for cell cycle progression, resulting in dramatic growth

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