05-1587

Anti-FOXO3a/FKHRL1 Antibody, clone 15F7.2

clone 15F7.2, from mouse

• 동의어(들): Forkhead box protein O3, AF6q21 protein, Forkhead in rhabdomyosarcoma-like 1
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: 15F7.2, monoclonal
• 종 반응성: human, rat
• 기술: immunohistochemistry: suitable; western blot: suitable
• 동형: IgG1κ
• NCBI 수납 번호: NP_001446
• UniProt 수납 번호: O43524
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... FOXO3(2309)

설명

일반 설명

Forkhead box protein O3 (FOXO3a), or FKHRL1, is a ubiquitous transcription factor that triggers the expression of pro-apoptotic genes under conditions of oxidative stress. FOXO3a is regulated by the Akt kinase which phosphorylates FOXO3a on serine 253, 315, and on threonine 32 residues. Modified FOXO3a is recognized by the 14-3-3 proteins which export FOXO3a to the cytoplasm, and consequently terminates its transcriptional activity. FOXO3a may also be regulated by lysine methylation in neuronal cells.

면역원

GST-tagged recombinant protein corresponding to human FOX03a.

애플리케이션

Anti-FOXO3a/FKHRL1 Antibody, clone 15F7.2 is a Mouse Monoclonal Antibody for detection of FOXO3a/FKHRL1 also known as Forkhead box protein O3, AF6q21 protein, Forkhead in rhabdomyosarcoma-like 1 & has been validated in WB & IHC.

Immunohistochemistry Analysis: A 1:300 dilution from a representative lot detected FOXO3a/FKHRL1 in human thyroid gland tissue and human breast cancer tissue.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Apoptosis - Additional

품질

Evaluated by Western Blot in rat liver tissue lysate.

Western Blot Analysis: 0.2 µg/mL of this antibody detected FOXO3a/FKHRL1 in 10 mg of rat liver tissue lysate.

표적 설명

~71 kDa observed. An uncharacterized band may be observed at ~28 kDa in some tissue lysates.

결합

Replaces: 04-1007

물리적 형태

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

분석 메모

Control
Rat liver tissue lysate.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable