05-1413
Anti-CD45RA Antibody, Clone MEM 56
clone MEM 56, from mouse
동의어(들):
CD45 antigen, Leukocyte common antigen precursor, SCID due to PTPRC deficiency, T200 glycoprotein, T200 leukocyte common antigen, Human homolog of severe combined immunodeficiency due to PTPRC deficiency, Leukocyte-common antigen, Protein tyrosine phosph
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모든 사진(4)
About This Item
UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41
추천 제품
생물학적 소스
mouse
Quality Level
항체 형태
purified immunoglobulin
항체 생산 유형
primary antibodies
클론
MEM 56, monoclonal
종 반응성
mouse
종 반응성(상동성에 의해 예측)
human, rat
기술
flow cytometry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable
동형
IgG2b
NCBI 수납 번호
UniProt 수납 번호
배송 상태
wet ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... PTPRC(5788)
관련 카테고리
일반 설명
CD45 is a receptor-tyrosine phosphatase required for antigen-induced signaling in T-cells and B-cells; its substrates are unknown. Interestingly, it associates with several Src-family tyrosine kinases, which are inhibited by tyrosine phosphorylation in their c-termini, suggesting a mechanism for signaling by CD45.
특이성
This antibody recognizes the 220 kDa molecular weight component of the leucocyte common antigen complex expressed on some CD4+ T lymphocytes, B lymphocytes and CD8+ lymphocytes. The CD45R+/CD4+ lymphocytes functionally exert suppressor/inducer activity in in vitro assay systems.
Antigen distribution:
B-cells (lg+) >95%
T-cells (E+) 70 + 10%
Peripheral blood lymphocytes 68 + 8%
T-cells (CD4+) 50 + 10%
Monocytes 50 + 20%
Thymocytes <5%
Granulocytes <1%
Antigen distribution:
B-cells (lg+) >95%
T-cells (E+) 70 + 10%
Peripheral blood lymphocytes 68 + 8%
T-cells (CD4+) 50 + 10%
Monocytes 50 + 20%
Thymocytes <5%
Granulocytes <1%
애플리케이션
Anti-CD45RA Antibody, Clone MEM 56 detects level of CD45RA & has been published & validated for use in FC, IF, IH(P), IP & WB.
Immunofluorescence: Identification of CD4+ suppressor/inducer cells, by indirect immunofluorescence staining.
Immunoprecipitation: A previous lot of this antibody detected 220 kDa molecular weight component of the leucocyte common antigen complex in immunoprecipitation.
Flow Cytometry: Purification or depletion of CD4+ suppressor/inducer cells by cell sorting or the panning techniques was performed on a previous lot.
Immunohistochemistry(paraffin): Representative testing from a previous lot.
Identification of CD45R+ lymphocytes in cryostat sections of frozen tissue and in formalin fixed wax embedded tissue.
Optimal Staining of CD45RA Monoclonal Antibody: Tonsil
Immunoprecipitation: A previous lot of this antibody detected 220 kDa molecular weight component of the leucocyte common antigen complex in immunoprecipitation.
Flow Cytometry: Purification or depletion of CD4+ suppressor/inducer cells by cell sorting or the panning techniques was performed on a previous lot.
Immunohistochemistry(paraffin): Representative testing from a previous lot.
Identification of CD45R+ lymphocytes in cryostat sections of frozen tissue and in formalin fixed wax embedded tissue.
Optimal Staining of CD45RA Monoclonal Antibody: Tonsil
Research Category
Signaling
Signaling
Research Sub Category
Immunological Signaling
Immunological Signaling
품질
Routinely evaluated by Western Blot on RAW 264 lysates.
Western Blot Analysis: 1:500 dilution of this lot detected CD45RA on 10 μg of RAW 264
Western Blot Analysis: 1:500 dilution of this lot detected CD45RA on 10 μg of RAW 264
표적 설명
~220 kDa
결합
Replaces: 04-1102
물리적 형태
Format: Purified
Purified
Purified mouse monoclonal IgG2b in phosphate buffered saline containing 10 mM sodium azide and 1 mg/mL bovine serum albumin.
We recommend that each laboratory determine an optimum working titre for use in its particular application.
We recommend that each laboratory determine an optimum working titre for use in its particular application.
저장 및 안정성
For use within 1 month of date of purchase store at 2-8°C, for long term storage aliquot antibody into small volumes and store at -20°C for up to 1 year from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
분석 메모
Control
RAW264 Cell Lysate
RAW264 Cell Lysate
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Xiangjun Liu et al.
Nature communications, 13(1), 1158-1158 (2022-03-05)
Cutaneous T cell lymphoma (CTCL) represents a heterogeneous group of non-Hodgkin lymphoma distinguished by the presence of clonal malignant T cells. The heterogeneity of malignant T cells and the complex tumor microenvironment remain poorly characterized. With single-cell RNA analysis and
Gaby J Ritfeld et al.
Cell transplantation, 24(11), 2209-2220 (2015-01-13)
The ability of intraspinal bone marrow stromal cell (BMSC) transplants to elicit repair is thought to result from paracrine effects by secreted trophic factors including brain-derived neurotrophic factor (BDNF). Here we used gene therapy to increase or silence BDNF production
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