05-1403

Anti-Giα2 Antibody, clone L5

clone L5, from mouse

• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: L5, monoclonal
• 종 반응성: guinea pig (43%), rat (69%), bovine (100%), human (62%), mouse (69%)
• 기술: immunohistochemistry: suitable (paraffin); western blot: suitable
• 동형: IgG2b
• NCBI 수납 번호: NM_002070.2
• UniProt 수납 번호: P04899
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... GNAI2(2771)

설명

특이성

Specificity confirmed by Western blot versus bovine Gαt, rat Goα, rat Giα-1, Giα-2, rat Gia-3, bovine Gsα, and murine GoαA and GoαB.

This antibody recognizes Giα-2.

면역원

Recombinant Giα-2, purified by the method of Linder (J. Biol. Chem. [1990] 265, 8243).

애플리케이션

Optimal working dilutions must be determined by end user.

Immunohistochemistry(paraffin): Representative testing from a previous lot.
Optimal Staining of G-Protein Monoclonal Antibody: Testicular Cancer

Western Blot: A previous lot of this antibody was used at 1:500 - 1:1000, with detection by 125l-labeled anti-IgG. Labels a single protein band of 39 - 42 kDa in bovine and rat membranes

Research Category
Signaling

Research Sub Category
GPCR, cAMP/cGMP & Calcium Signaling

This Anti-Giα2 Antibody, clone L5 is validated for use in WB, IH(P) for the detection of Giα2.

품질

Routinely evaluated by Western Blot on Huvec lysates.

Western Blot Analysis: 1:500 dilution of this lot detected G I ALPHA 2 on 10 μg of Huvec lysates.

표적 설명

Labels a single protein band of 39-42 kDa in bovine and rat membranes.

결합

Replaces: MAB3077

물리적 형태

Format: Purified

Purified

Purified mouse monoclonal IgG2b, in 0.02 M phosphate buffer, 0.25 M sodium chloride, pH 7.6, with 0.1% sodium azide.

저장 및 안정성

Maintain at 2-8°C for 1 year from date of receipt.

분석 메모

Control
Huvec cell lysate

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable