추천 제품
생물학적 소스
mouse
Quality Level
항체 형태
purified immunoglobulin
항체 생산 유형
primary antibodies
클론
3F2, monoclonal
종 반응성
mouse, human, canine, rat
제조업체/상표
Upstate®
기술
ELISA: suitable
immunoprecipitation (IP): suitable
western blot: suitable
동형
IgG1
UniProt 수납 번호
배송 상태
wet ice
타겟 번역 후 변형
phosphorylation (pThr654)
유전자 정보
dog ... Egfr(404306)
human ... EGFR(1956)
mouse ... Egfr(13649)
rat ... Egf(25313)
특이성
Recognizes phosphorylated EGFR on Threonine 654
면역원
KLH-conjugated synthetic peptide encompassing the surrounding amino acids of Thr 654 in human EGFR
애플리케이션
Detect phospho-EGFR (Thr654) using this Anti-phospho-EGFR (Thr654) Antibody, clone 3F2 validated for use in ELISA, IP & WB.
품질
Routinely evaluated by immunoblot.
표적 설명
180 kDa
물리적 형태
100 µg (0.1 mg/ml) of mouse monoclonal IgG1 in PBS containing 0.09% sodium azide, PEG, sucrose and 50% glycerol
Format: Purified
분석 메모
Control
Includes PMA and pervanadate treated Hep2G cell lysate as a positive control
Includes PMA and pervanadate treated Hep2G cell lysate as a positive control
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
법적 정보
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
10 - Combustible liquids
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Protein kinase C phosphorylation of the EGF receptor at a threonine residue close to the cytoplasmic face of the plasma membrane
Nature, 311, 480-483 null
Phosphorylation of Thr654 but not Thr669 within the juxtamembrane domain of the EGF receptor inhibits calmodulin binding
Biochemical and biophysical research communications, 347, 381-387 (2006)
The Journal of biological chemistry, 288(36), 26323-26334 (2013-07-31)
Ligand-induced ubiquitylation of EGF receptor (EGFR) is an important regulatory mechanism that controls endocytic trafficking of the receptor and its signaling potential. Here we report that tetraspanin CD82/KAI1 specifically suppresses ubiquitylation of EGFR after stimulation with heparin-binding EGF or amphiregulin
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