추천 제품
Quality Level
형태
liquid
제조업체/상표
Upstate®
농도
2 M
기술
activity assay: suitable (reverse transcriptase)
배송 상태
dry ice
일반 설명
Features & Advantages
In Vivo SHAPE Reagent is used for the determination of RNA structure. This reagent contains a highly pure form of 2-methylnicotinic acid imidazolide (NAI). This compound forms 2’-O-adducts on single stranded regions of RNA that can be detected by Selective 2’-Hydroxyl Acylation and Primer Extension (SHAPE).
In contrast to other reagents used for the SHAPE method, the NAI–based In Vivo SHAPE Reagent is rapidly absorbed into cells and is formulated to have low levels of toxicity making it ideal for use with live cells. Unlike other reagents used for SHAPE analysis, In Vivo SHAPE Reagent reacts in an unbiased fashion with all four RNA nucleotides. This combination of low toxicity, rapid uptake and lack of nucleotide bias enables high resolution RNA structure analysis in a variety of living systems.
- Ready-to-use RNase-free solution
- Low toxicity allows use with live cells
- High cell-permeability for rapid modification of RNA
- Reacts with all RNA nucleotides without bias for high resolution mapping
In Vivo SHAPE Reagent is used for the determination of RNA structure. This reagent contains a highly pure form of 2-methylnicotinic acid imidazolide (NAI). This compound forms 2’-O-adducts on single stranded regions of RNA that can be detected by Selective 2’-Hydroxyl Acylation and Primer Extension (SHAPE).
In contrast to other reagents used for the SHAPE method, the NAI–based In Vivo SHAPE Reagent is rapidly absorbed into cells and is formulated to have low levels of toxicity making it ideal for use with live cells. Unlike other reagents used for SHAPE analysis, In Vivo SHAPE Reagent reacts in an unbiased fashion with all four RNA nucleotides. This combination of low toxicity, rapid uptake and lack of nucleotide bias enables high resolution RNA structure analysis in a variety of living systems.
Folding into higher-order structures allows RNAs to function inside living cells and form dynamic complexes with effector proteins. Predicting these 3D structures is challenging and therefore a number of probing techniques have been developed to study RNA folding.
Compounds such as 2-methylnicotinic acid imidazolide (NAI) contained the In Vivo SHAPE Reagent result in the formation of 2-O-adducts. These adducts form rapidly on single stranded unpaired RNA and much more slowly on based paired (double stranded) RNA. Modified RNA can then be analyzed by primer extension. Modified nucleotides are not extended by RNA dependent DNA polymerases thus allowing sites of modification to be detected.
Compounds such as 2-methylnicotinic acid imidazolide (NAI) contained the In Vivo SHAPE Reagent result in the formation of 2-O-adducts. These adducts form rapidly on single stranded unpaired RNA and much more slowly on based paired (double stranded) RNA. Modified RNA can then be analyzed by primer extension. Modified nucleotides are not extended by RNA dependent DNA polymerases thus allowing sites of modification to be detected.
성분
2-methylnicotinic acid imidazolide (NAI) in DMSO
품질
Purity of NAI compound is tested by HPLC.
물리적 형태
0.5 mL vial containing 2M NAI (>97% pure) in DMSO solution
2M NAI in DMSO
저장 및 안정성
Store NAI at -20°C in a non-frost-free freezer protected from light. With proper storage (protect from light and avoid excessive freeze-thaw cycles) this product is stable for up to 6 months from the date of receipt.
법적 정보
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
관련 제품
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Kevin A Wilkinson et al.
Nature protocols, 1(3), 1610-1616 (2007-04-05)
Selective 2'-hydroxyl acylation analyzed by primer extension (SHAPE) interrogates local backbone flexibility in RNA at single-nucleotide resolution under diverse solution environments. Flexible RNA nucleotides preferentially sample local conformations that enhance the nucleophilic reactivity of 2'-hydroxyl groups toward electrophiles, such as
Robert C Spitale et al.
Nature chemical biology, 9(1), 18-20 (2012-11-28)
RNA structure has important roles in practically every facet of gene regulation, but the paucity of in vivo structural probes limits current understanding. Here we design, synthesize and demonstrate two new chemical probes that enable selective 2'-hydroxyl acylation analyzed by
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