03-209

SET7/9 human recombinant - Methyltransferase enzyme

Human recombinant SET7/9 expressed in E. Coli.

• UNSPSC 코드: 12352200
• NACRES: NA.54

속성

• 생물학적 소스: human
• Quality Level: 100
• 재조합: expressed in E. coli
• 분석: >95% (SDS-PAGE)
• 분자량: ~41 kDa
• 포장: pkg of 100 μg
• 제조업체/상표: Upstate^®
• 저장 조건: avoid repeated freeze/thaw cycles
• 농도: 1 mg/mL
• 기술: enzyme immunoassay: suitable
• UniProt 수납 번호: Q8WTS6

설명

일반 설명

The methylation of histones plays a pivotal role in the regulation of chromatin structure and gene expression. Histone methylation can occur on Arg or Lys residues, with an exquisite site selectivity for Lys methylation at specific positions in the N-termini of histones H3 and H4. SET7/9, a histone methyltransferase (HMTase), which transfers methyl groups to Lys4 of Histone H3, forms a complex with S-adenosyl-L-methionine. This complex contains an active site consisting of a binding pocket where an AdoMet molecule, in an unusual conformation, binds a narrow substrate-specific channel that only unmethylated lysine residues can access, and a catalytic tyrosine residue.

특이성

<1.0EU/µg protein (LAL test)

애플리케이션

Human recombinant SET7/9 expressed in E. Coli.

물리적 형태

Format: Purified

In liquid containing 50 mM Tris-HCl, pH 7.5, containing 0.2 M sodium chloride, 5 mM DTT and 20% glycerol. Frozen solution.

저장 및 안정성

Stable for 12 months at -20°C from date of receipt. Avoid multiple freeze/thaw cycles.

분석 메모

Methyltransferase assays

법적 정보

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1