추천 제품
형태
tablet
사용
sufficient for 50 mL (05892953001)
sufficient for 50 mL (06538282001)
포장
pkg of 2 × 10 tablets (05892953001)
pkg of 6 × 10 tablets (06538282001)
제조업체/상표
Roche
solubility
aqueous buffer: soluble
흡수
0.4 at 280 nm (1x solution)
저장 온도
2-8°C
일반 설명
cOmplete ULTRA Tablets, EDTA-free contain the most powerful protease inhibitor cocktail for the optimal protection of your proteins against protease degradation. One cOmplete ULTRA Tablet, EDTA-free is sufficient for a volume of 50 ml extraction solution. cOmplete ULTRA Tablets contain both irreversible and reversible protease inhibitors. Tablets are supplied in glass vials. Alternatively, these tablets are also available in EASYpack blister packs. EASYpacks provide the most convenient form of delivery - single tablets are easily pushed through the blister foil directly into the buffer.
cOmplete ULTRA Tablets EDTA-free are identical to cOmplete ULTRA Tablets, the only difference being that no EDTA or other chelating agents (e.g. EGTA) are included.
Contents
Tablets in a glass vial. Each tablet is sufficient for a volume of 50ml extraction solution.
cOmplete ULTRA Tablets EDTA-free are identical to cOmplete ULTRA Tablets, the only difference being that no EDTA or other chelating agents (e.g. EGTA) are included.
Contents
Tablets in a glass vial. Each tablet is sufficient for a volume of 50ml extraction solution.
특이성
Inhibits serine proteases, cysteine proteases. Does not inhibit metalloproteases.
애플리케이션
During cell lysis and purification, target proteins are at risk of being cleaved by proteases, which are naturally present in cells. To ensure that full-length proteins are obtained, cOmplete ULTRA Tablets are used for the inhibition of an extended range of proteases. This new formulation shows high inhibition ratios due to its broadened range of inhibitors compared to standard cOmplete Tablets.
This new protease inhibitor cocktail now inhibits aspartic proteases, as well as serine and cysteine proteases. cOmplete ULTRA Tablets were developed for improved efficiency on different cell types. Best performance is guaranteed in lysates of bacteria, yeast, insect cells, and a wide range of higher cell lines.
This tablet does not contain EDTA; therefore metalloproteases are not inhibited.
This new protease inhibitor cocktail now inhibits aspartic proteases, as well as serine and cysteine proteases. cOmplete ULTRA Tablets were developed for improved efficiency on different cell types. Best performance is guaranteed in lysates of bacteria, yeast, insect cells, and a wide range of higher cell lines.
This tablet does not contain EDTA; therefore metalloproteases are not inhibited.
cOmplete™ ULTRA Tablets, EDTA-free, glass vials Protease Inhibitor Cocktail has been used as a component of
- ice-cold radioimmunoprecipitation assay buffer (RIPA) for the lysis of retinal ganglion cells
- lysis buffer for green fluorescent protein (GFP)- cell-penetrating peptides (CPPs) fusion protein isolation from E coli
- homogenization buffer for mice protein isolation from intestinal tissue
특징 및 장점
- Protect your precious proteins.
- Why settle for less.
- Defend against a broad range of proteases.
- Convenient and safe tablet format.
into your buffers.
성분
Proprietary mixture of several protease inhibitors with broad inhibitory specificity.
물리적 형태
Tablets in a glass vial. Each tablet is sufficient for a volume of 50 ml extraction solution.
제조 메모
Working concentration: 1 Tablet per 50 ml extraction solution.
Working solution: Handling
Carefully push the tablet through the foil packaging using the base of your thumb (not your fingernail) to prevent the breakage of tablets.
Preparation of Working Solution
One tablet is sufficient for the inhibition of proteolytic activity in 50 ml extraction solution. If very high proteolytic activity is present, 2-3 tablets should be used for 50 ml extraction buffer. The tablets can be added directly to the extraction medium.
Stock Solution (2x conc.)
Alternatively to the direct use with lysates, a 2x stock solution can be prepared, with the following procedure:
• Add one cOmplete ULTRA Tablet, EDTA-free to 25 ml double-distilled water in a PE or PP tube.
• Vortex 4 min, until the tablet is dissolved.
• The stock solution is ready to use even if there are very fine particles still visible in solution. These particles do not interfere with the inhibition performance of the tablets.
• This stock solution is stable for 4 weeks, stored at 2 to 8 °C, and for at least 4 weeks at -15 to -25 °C as well.
• During storage of the 2x stock solution, the particles can sediment. Briefly vortex the stock solution prior to use.
Storage conditions (working solution): The stock solution in water is stable for 4 weeks, stored at 2 to 8 °C, and for at least 4 weeks at -15 to -25 °C as well.
Working solution: Handling
Carefully push the tablet through the foil packaging using the base of your thumb (not your fingernail) to prevent the breakage of tablets.
Preparation of Working Solution
One tablet is sufficient for the inhibition of proteolytic activity in 50 ml extraction solution. If very high proteolytic activity is present, 2-3 tablets should be used for 50 ml extraction buffer. The tablets can be added directly to the extraction medium.
Stock Solution (2x conc.)
Alternatively to the direct use with lysates, a 2x stock solution can be prepared, with the following procedure:
• Add one cOmplete ULTRA Tablet, EDTA-free to 25 ml double-distilled water in a PE or PP tube.
• Vortex 4 min, until the tablet is dissolved.
• The stock solution is ready to use even if there are very fine particles still visible in solution. These particles do not interfere with the inhibition performance of the tablets.
• This stock solution is stable for 4 weeks, stored at 2 to 8 °C, and for at least 4 weeks at -15 to -25 °C as well.
• During storage of the 2x stock solution, the particles can sediment. Briefly vortex the stock solution prior to use.
Storage conditions (working solution): The stock solution in water is stable for 4 weeks, stored at 2 to 8 °C, and for at least 4 weeks at -15 to -25 °C as well.
재구성
Soluble in aqueous buffers, or add directly to extraction media. For the stock solution, use water only.
기타 정보
For life science research only. Not for use in diagnostic procedures.
법적 정보
cOmplete is a trademark of Roche
신호어
Danger
유해 및 위험 성명서
Hazard Classifications
Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1B
Storage Class Code
8A - Combustible, corrosive hazardous materials
WGK
WGK 2
Flash Point (°F)
does not flash
Flash Point (°C)
does not flash
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
이미 열람한 고객
Ezh2 does not mediate retinal ganglion cell homeostasis or their susceptibility to injury
Cheng L, et al.
Testing, 13(2), e0191853-e0191853 (2018)
Cell-penetrating peptide sequence and modification dependent uptake and subcellular distribution of green florescent protein in different cell lines
Patel SG, et al.
Scientific Reports, 9(1), 6298-6298 (2019)
Strain specific maturation of Dendritic cells and production of IL-1beta controls CD40-driven colitis
Wagner AO, et al.
PLoS ONE, 14(1), e0210998-e0210998 (2019)
George E Gentsch et al.
Cold Spring Harbor protocols, 2014(5), doi:10-doi:10 (2014-05-03)
Chromatin immunoprecipitation (ChIP) combined with genomic analysis techniques provide a global snapshot of protein-DNA interactions in the context of chromatin, yielding insights into which genomic loci might be regulated by the DNA-associated protein under investigation. This protocol describes how to
Barbara Mosca et al.
Nature communications, 4, 1541-1541 (2013-02-28)
Muscle strength declines with age in part due to a decline of Ca(2+) release from sarcoplasmic reticulum calcium stores. Skeletal muscle dihydropyridine receptors (Ca(v)1.1) initiate muscle contraction by activating ryanodine receptors in the sarcoplasmic reticulum. Ca(v)1.1 channel activity is enhanced
문서
Get maximum protection after protein isolation by using protease and phosphatase inhibitor cocktail tablets from Roche.
관련 콘텐츠
Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.
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