LC/MS Analysis of Glyphosate and Metabolites on apHera™ NH2, 2 mm I.D. Column
Materials
analytical column
Product No.
Description
Pricing
accessory
Product No.
Description
Pricing
Certified Vial Kit, Low Adsorption (LA), 2 mL, pk of 100
volume 2 mL, amber glass vial (with marking spot), natural PTFE/silicone septa (with slit), thread for 9 mmCONDITIONS
column
apHera NH2, 15 cm x 2 mm I.D., 5 μm particles (56400AST)
mobile phase
[A] water; [B] 20mM ammonium carbonate pH 9.0
gradient
100%A for 0.5 min; to 100% B in 0.1 min; held at 100% B for 6.4 min; to 100% A in 0.1 min; held at 100% A for 3 min
flow rate
0.3 mL/min
pressure
1570 psi (108 bar)
column temp.
30 °C
detector
ESI-MS(-); MS/MS 168/63; 110/63; 180/63
injection
15 μL
sample
50 ng/mL each compound in 50:50 methanol:water with 0.05% formic acid
Description
Analysis Note
An LC/MS method for analysis of glyphosate, AMPA and glufosinate using MS-compatible mobile phase on an ion-exchange pH-stable HPLC column with mass spectrometry detection is shown here.
Legal Information
apHera is a trademark of Sigma-Aldrich Co. LLC