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  • A new type of protein lysine methyltransferase trimethylates Lys-79 of elongation factor 1A.

A new type of protein lysine methyltransferase trimethylates Lys-79 of elongation factor 1A.

Biochemical and biophysical research communications (2014-12-03)
Maria C Dzialo, Kyle J Travaglini, Sean Shen, Joseph A Loo, Steven G Clarke
ABSTRACT

The elongation factors of Saccharomyces cerevisiae are extensively methylated, containing a total of ten methyllysine residues. Elongation factor methyltransferases (Efm1, Efm2, Efm3, and Efm4) catalyze at least four of these modifications. Here we report the identification of a new type of protein lysine methyltransferase, Efm5 (Ygr001c), which was initially classified as N6-adenine DNA methyltransferase-like. Efm5 is required for trimethylation of Lys-79 on EF1A. We directly show the loss of this modification in efm5Δ strains by both mass spectrometry and amino acid analysis. Close homologs of Efm5 are found in vertebrates, invertebrates, and plants, although some fungal species apparently lack this enzyme. This suggests possible unique functions of this modification in S. cerevisiae and higher eukaryotes. The misannotation of Efm5 was due to the presence of a DPPF sequence in post-Motif II, typically associated with DNA methylation. Further analysis of this motif and others like it demonstrates a potential consensus sequence for N-methyltransferases.

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Product Description

Lysine hydrochloride, European Pharmacopoeia (EP) Reference Standard
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L-Lysine, ≥98% (TLC)
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L-Lysine acetate salt, ≥98% (HPLC)
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L-Lysine, crystallized, ≥98.0% (NT)
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L-Lysine, analytical standard
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Lysine acetate, European Pharmacopoeia (EP) Reference Standard
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L-Lysine monohydrochloride, BioUltra, ≥99.5% (AT)
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L-Lysine monohydrochloride, reagent grade, ≥98% (HPLC)
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Nε,Nε,Nε-Trimethyllysine hydrochloride, ≥97% (TLC)
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L-Lysine monohydrochloride, from non-animal source, meets EP, JP, USP testing specifications, suitable for cell culture, 98.5-101.0%
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L-Lysine monohydrochloride, certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland
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L-Lysine monohydrochloride, Pharmaceutical Secondary Standard; Certified Reference Material
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L-Lysine Acetate, Pharmaceutical Secondary Standard; Certified Reference Material
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