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Key Documents

MAK036

Sigma-Aldrich

Hemin Assay Kit

sufficient for 100 colorimetric tests

Synonym(s):

Hemin Quantitative Test Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 100 colorimetric tests

application(s)

cosmetics
food and beverages

detection method

colorimetric

relevant disease(s)

immunological diseases; rheumatological diseases; gastrointestinal diseases; cardiovascular diseases

storage temp.

2-8°C

General description

Hemin, the oxidized version of heme, is an iron-containing prosthetic group for a diverse group of proteins. Free heme, which can be released from hemoglobin following hemolysis, is pro-inflammatory and contributes to iron-derived reactive oxygen species. Free hemin levels may be upregulated in various pathological conditions and can contribute to various inflammatory conditions including vascular disorders, renal failure, and immune-mediated disorders.

Application

Hemin Assay Kit has been used to determine the level of free hemin (oxidized heme).

Suitability

Suitable for use with cell and tissue culture supernatants, urine, plasma, serum, fecal material, media, and other biological fluids.

Principle

In this assay, Hemin concentration is determined by a coupled enzyme reaction, which results in a colorimetric (570 nm) product, proportional to the Hemin present.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Resp. Sens. 1

Storage Class Code

10 - Combustible liquids

Flash Point(F)

188.6 °F - closed cup

Flash Point(C)

87 °C - closed cup


Certificates of Analysis (COA)

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