Hexanucleotide primers are a mixture of random 5′-hydroxyl hexanucleotides or hexamers, and can be used to quickly and efficiently prepare radioactive or non-radioactive probes using a DNA polymerase and a suitable DNA template.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 23(16), 6404-6412 (2003-07-25)
Multiple evidence suggests that fibroblast growth factors (FGFs), most prominently FGF-2, affect astroglial proliferation, maturation, and transition to a reactive phenotype in vitro, and after exogenous administration, in vivo. Whether this reflects a physiological role of endogenous FGF is unknown.
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 10-10 (1989)
The Journal of neuroscience : the official journal of the Society for Neuroscience, 23(5), 1792-1803 (2003-03-12)
The mammalian olfactory epithelium (OE) is composed of primary olfactory sensory neurons (OSNs) that are renewed throughout adulthood by local, restricted neuronal progenitor cells. The molecular signals that control this neurogenesis in vivo are unknown. Using olfactory bulb ablation (OBX)
A technique for conveniently radiolabeling DNA restriction endonuclease fragments to high specific activity is described. DNA fragments are purified from agarose gels directly by ethanol precipitation and are then denatured and labeled with the large fragment of DNA polymerase I
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