402085
Indirubin-3′-monoxime
A potent, reversible, and ATP-compatible inhibitor of GSK-3β (glycogen synthase kinase 3β), Cdk1 (cyclin-dependent kinase1) and Cdk5 (IC₅₀ = 22 nM, 180 nM, and 100 nM, respectively).
Synonym(s):
Indirubin-3′-monoxime
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About This Item
Empirical Formula (Hill Notation):
C16H11N3O2
CAS Number:
Molecular Weight:
277.28
MDL number:
UNSPSC Code:
12352200
NACRES:
NA.77
Quality Level
Assay
≥98% (HPLC)
form
solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
protect from light
color
dark red
solubility
DMSO: 5 mg/mL
shipped in
ambient
storage temp.
−20°C
General description
A potent cell-permeable, reversible, and ATP-compatible inhibitor of glycogen synthase kinase-3β (IC50 = 22 nM) and cyclin-dependent kinases (IC50 = 180 nM for Cdk1 and 100 nM for Cdk5). Inhibits the proliferation of a large range of cells by arresting them in the G2/M phase of the cell cycle. Inhibits Tau phosphorylation in vitro and in vivo at Alzheimer′s disease-specific sites. Also reported to inhibit the in vivo phosphorylation of DARPP-32 by Cdk5 on Thr75.
A potent, reversible, and ATP-compatible inhibitor of GSK-3β (glycogen synthase kinase 3β), Cdk1 (cyclin-dependent kinase1) and Cdk5 (IC50 = 22 nM, 180 nM, and 100 nM, respectively). Inhibits the proliferation of a large range of cells by arresting them in the G2/M phase of the cell cycle. Inhibits Tau phosphorylation in vitro and in vivo at Alzheimer’s disease-specific sites. Also reported to inhibit the in vivo phosphorylation of DARPP-32 by Cdk5 on Thr75.
Biochem/physiol Actions
Cell permeable: yes
Primary Target
Gsk-3β
Gsk-3β
Product competes with ATP.
Reversible: yes
Target IC50: 22 nM, 180 nM, and 100 nM against GSK-3β, Cdk1, and Cdk5, respectively
Packaging
Packaged under inert gas
Warning
Toxicity: Standard Handling (A)
Reconstitution
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Other Notes
Bain, J., et al. 2003. Biochem. J. 371, 199.
Leclerc, S., et al. 2000. J. Biol. Chem.276, 251.
Hoessel, R., et al. 1999. Nat. Cell Biol. 1, 60.
Leclerc, S., et al. 2000. J. Biol. Chem.276, 251.
Hoessel, R., et al. 1999. Nat. Cell Biol. 1, 60.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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