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TP0300

Sigma-Aldrich

Total Protein Kit, Micro Lowry, Peterson′s Modification

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

usage

sufficient for ~200 assays

General description

This method utilizes sodium dodecylsulfate, included in the Lowry reagent, to facilitate the dissolution of relatively insoluble lipoproteins. An optional deoxycholate-TCA precipitation step permits removal of interfering substances such as Tris, sucrose, EDTA, salts, etc.

Application

Total Protein Kit, Micro Lowry, Peterson′s Modification has been used to measure protein content.

Features and Benefits

  • Complete kit for protein assays
  • Most widely cited protein assay
  • Less protein to protein variation than other assays
  • Contains reagents that can remove interfering substances

Principle

An optional deoxycholate-TCA precipitation step permits removal of interfering substances such as Tris, sucrose, EDTA, salts, etc. Absorbance at 500-800 nm (typically read at 750 nm) is used to determine protein concentration from a standard curve.

Signal Word

Danger

Hazard Statements

H290,H302,H314,H335,H410

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

8A - Combustible corrosive hazardous materials

WGK

WGK 3

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Protective effect of carbon monoxide pre-conditioning on LPS-induced endothelial cell stress
Bernardini C, et al.
Cell Stress & Chaperones, 15(2), 219-224 (2010)
Dalia A Elgamal et al.
Molecular and cellular endocrinology, 503, 110700-110700 (2020-01-07)
Genetic and epigenetic factors contribute equally to the pathogenesis of type 1 diabetes mellitus. Sodium butyrate (NaB) has been reported to improve glucose homeostasis by modulation of the p38/ERK MAPK pathway. This work aims to evaluate the effect of NaB
Chiara Bernardini et al.
BMC complementary and alternative medicine, 18(1), 194-194 (2018-06-27)
It is widely accepted the key role of endothelium in the onset of many chronic and acute vascular and cardiovascular diseases. In the last decade, traditional compounds utilized in "folk medicine" were considered with increasing interest to discover new bioactive
Expression of alpha-gustducin and alpha-transducin, G proteins coupled with taste receptors, in boar sperm
Spinaci M, et al.
Theriogenology, 82(1), 144-151 (2014)
Aberrant glycosylation of alphavbeta3 integrin is associated with melanoma progression
Pochec E, et al.
Anticancer Research, 35(4), 2093-2103 (2015)
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Protocols

Protein Determination by the Warburg-Christian Method

To determine protein content, the Warburg-Christian method refers to measuring protein samples at 280 nm using a spectrophotometer.

Related Content

Protein Quantitation

Protein quantification methods, reagents, and immunoassay technology for accurately measuring the protein concentrations in a variety of samples.

Tools for Protein Quantitation

Products for traditional and alternative protein quantitation techniques available, including BCA, Bradford, Lowry, and more.

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